Purification notesab59997 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
ab59997 at 1/50-1/100 dilution staining RSK1 p90 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining
References for Anti-RSK1 p90 (phospho S364) antibody (ab59997)
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