Rabbit monoclonal [E238] to RSK1 p90 (phospho T359 + S363)
This antibody is specific for human RSK1 p90 phoshorylated on Threonine 359 and Serine 363.
80% identities with RSK3 p90 (pT365+pS369), 75% with RSK2 p90 (pT356+pS360) and 74% identities with RSK6 p90 (pT368+pS372)
dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
EC 126.96.36.199 antibody
HU 1 antibody
MAP kinase activated protein kinase 1a antibody
MAP kinase-activated protein kinase 1a antibody
MAPK-activated protein kinase 1a antibody
MAPKAP kinase 1a antibody
Mitogen-activated protein kinase-activated protein kinase 1A antibody
p90 RSK1 antibody
p90-RSK 1 antibody
Ribosomal protein S6 kinase 90kD 1 antibody
Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
Ribosomal protein S6 kinase alpha 1 antibody
Ribosomal protein S6 kinase alpha-1 antibody
Ribosomal protein S6 kinase polypeptide 1 antibody
Ribosomal S6 kinase 1 antibody
RPS6K1 alpha antibody
RSK 1 antibody
RSK 1 p90 antibody
S6K alpha 1 antibody
Western blot - Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 90 kDa
Exposure time : 16 minutes
BT549 cells were incubated at 37 °C for 1h with vehicle control (0 μM) and different concentrations of PD 173074 (ab141117) in DMSO. Decreased expression of RSK1 p90 (phospho T359 + S363) (ab32413) correlates with an increase in PD 173074 concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32413 at 1/5000 dilutionand ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
Western blot - RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)
All lanes : Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) at 1/20000 dilution
Lane 1 : A431 cell lysate untreated. Lane 2 : A431 cell lysate treated with EGF.
Predicted band size : 90 kDa Observed band size : 90 kDa