Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)

Overview

  • Product nameAnti-RSK1 p90 (phospho T359 + S363) antibody [E238]
    See all RSK1 p90 primary antibodies
  • Description
    Rabbit monoclonal [E238] to RSK1 p90 (phospho T359 + S363)
  • SpecificityThis antibody is specific for human RSK1 p90 phoshorylated on Threonine 359 and Serine 363. 80% identities with RSK3 p90 (pT365+pS369), 75% with RSK2 p90 (pT356+pS360) and 74% identities with RSK6 p90 (pT368+pS372)
  • Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RSK1 p90 aa 350-450.

  • Positive control
    • WB: A431 cell lysate. IHC-P: Human cervical carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab32413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/50.
IP 1/40.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications
      Autophosphorylated on Ser-380, as part of the activation process.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kDa ribosomal protein S6 kinase 1 antibody
      • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
      • dJ590P13.1 antibody
      • EC 2.7.11.1 antibody
      • HU 1 antibody
      • HU1 antibody
      • KS6A1_HUMAN antibody
      • MAP kinase activated protein kinase 1a antibody
      • MAP kinase-activated protein kinase 1a antibody
      • MAPK-activated protein kinase 1a antibody
      • MAPKAP kinase 1a antibody
      • MAPKAPK-1a antibody
      • MAPKAPK1A antibody
      • MGC79981 antibody
      • Mitogen-activated protein kinase-activated protein kinase 1A antibody
      • OTTHUMP00000004113 antibody
      • p90 RSK1 antibody
      • p90-RSK 1 antibody
      • p90rsk antibody
      • p90RSK1 antibody
      • p90S6K antibody
      • pp90RSK1 antibody
      • Ribosomal protein S6 kinase 90kD 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
      • Ribosomal protein S6 kinase alpha 1 antibody
      • Ribosomal protein S6 kinase alpha-1 antibody
      • Ribosomal protein S6 kinase polypeptide 1 antibody
      • Ribosomal S6 kinase 1 antibody
      • RPS6K1 alpha antibody
      • rps6ka antibody
      • Rps6ka1 antibody
      • RSK 1 antibody
      • RSK 1 p90 antibody
      • RSK antibody
      • RSK-1 antibody
      • RSK1 antibody
      • S6K alpha 1 antibody
      • S6K-alpha-1 antibody
      see all

    Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] images


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 90 kDa


      Exposure time : 16 minutes

      BT549 cells were incubated at 37 °C for 1h with vehicle control (0 μM) and different concentrations of PD 173074 (ab141117) in DMSO. Decreased expression of RSK1 p90 (phospho T359 + S363) (ab32413) correlates with an increase in PD 173074 concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32413 at 1/5000 dilutionand ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    • All lanes : Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) at 1/20000 dilution

      Lane 1 : A431 cell lysate untreated.
      Lane 2 : A431 cell lysate treated with EGF.


      Predicted band size : 90 kDa
      Observed band size : 90 kDa
    • Ab32413, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
      Left image : Untreated.
      Right image : Treated with Phosphatase.

    References for Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)

    This product has been referenced in:
    • Chen X  et al. Combined PKC and MEK inhibition in uveal melanoma with GNAQ and GNA11 mutations. Oncogene N/A:N/A (2013). Read more (PubMed: 24141786) »
    • Chia KM  et al. A Feedback Loop between Androgen Receptor and ERK Signaling in Estrogen Receptor-Negative Breast Cancer. Neoplasia 13:154-66 (2011). IHC-P . Read more (PubMed: 21403841) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Sample Human Tissue sections (Human fibromatosis)
    Specification Human fibromatosis
    Permeabilization Yes - Tween-20
    Fixative Paraformaldehyde
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    Submitted Dec 05 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated
    Sample Mouse Tissue sections (mouse liver)
    Specification mouse liver
    Permeabilization Yes - Tween-20
    Fixative Formaldehyde
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    Submitted Nov 25 2014

    Application Western blot
    Loading amount 75 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Pig Cell lysate - whole cell (PAE cells)
    Specification PAE cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Verified customer

    Submitted Mar 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"