Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RSK1 p90.
K562 cell lysate.
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Immunofluorescence staining of K562 cells with purified ab62324 at a working dilution of 1/500, counter-stained with DAPI. The cells were treated with 500 nM TPA for 15 minutes. Both the treated and untreated cells are shown. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the right hand panels. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom panels - for negative control 1, purified ab62324 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Western blot - RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/500 dilution (Unpurified)
Lane 1 : K562 cell lysate; untreated Lane 2 : K562 cell lysate; treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary goat anti-rabbit HRP conjugated, at 1/2000 dilution
Predicted band size : 83 kDa Observed band size : 83 kDa Beta Tubulin has been included as a loading control.
References for Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
This product has been referenced in:
Chaikuad A et al. A unique inhibitor binding site in ERK1/2 is associated with slow binding kinetics. Nat Chem Biol10:853-60 (2014).
Read more (PubMed: 25195011) »
Talbot AL et al. Aconitase regulation of erythropoiesis correlates with a novel licensing function in erythropoietin-induced ERK signaling. PLoS One6:e23850 (2011).
Read more (PubMed: 21887333) »