Anti-RSK3 antibody (ab227230)
Key features and details
- Rabbit polyclonal to RSK3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RSK3 antibody
See all RSK3 primary antibodies -
Description
Rabbit polyclonal to RSK3 -
Host species
Rabbit -
Specificity
Based on sequence alignment of the used immunogen, this antibody may cross-react with RSK2. However, we have no experimental testing data for cross-reactivity of ab227230 with other RSK proteins.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment within Human RSK3 (internal sequence). The exact sequence is proprietary.
Database link: Q15349 -
Positive control
- WB: Mouse brain lysate; HepG2 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 83 kDa. |
Target
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Function
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells. -
Tissue specificity
Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsActivated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1.
N-terminal myristoylation results in an activated kinase in the absence of added growth factors. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 517953 Cow
- Entrez Gene: 6196 Human
- Entrez Gene: 20112 Mouse
- Entrez Gene: 117269 Rat
- Omim: 601685 Human
- SwissProt: Q15349 Human
- SwissProt: Q9WUT3 Mouse
- Unigene: 655277 Human
see all -
Alternative names
- 90 kDa ribosomal protein S6 kinase 2 antibody
- HU 2 antibody
- KS6A2_HUMAN antibody
see all
Images
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Anti-RSK3 antibody (ab227230) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 83 kDa7.5% SDS-PAGE gel.
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Anti-RSK3 antibody (ab227230) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 83 kDa7.5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227230 has not yet been referenced specifically in any publications.