Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)

Overview

  • Product nameAnti-RSK4 (phospho S232) antibody [EP1524Y]
    See all RSK4 primary antibodies
  • Description
    Rabbit monoclonal [EP1524Y] to RSK4 (phospho S232)
  • SpecificityDue to high sequence homology, this antibody might cross-react with RSK1(Q15418) and RSK3(P51812).
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RSK4 aa 200-300 (phospho S232).
    Database link: Q9UK32

  • Positive control
    • Hela cell lysate and human muscle tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP1524Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications
      Autophosphorylated on Ser-389, as part of the activation process.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kDa ribosomal protein S6 kinase 6 antibody
      • KS6A6_HUMAN antibody
      • p90 RSK 6 antibody
      • p90-RSK 6 antibody
      • p90RSK6 antibody
      • pp90RSK4 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 6 antibody
      • Ribosomal protein S6 kinase alpha 6 antibody
      • Ribosomal protein S6 kinase alpha-6 antibody
      • Ribosomal S6 kinase 4 antibody
      • rps6ka6 antibody
      • RSK 4 antibody
      • RSK-4 antibody
      • S6K-alpha-6 antibody
      see all

    Anti-RSK4 (phospho S232) antibody [EP1524Y] images

    • All lanes : Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290) at 1/10000 dilution

      Lane 1 : Hela cell lysate untreated
      Lane 2 : Hela cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled anti-rabbit at 1/2000 dilution

      Predicted band size : 84 kDa
      Observed band size : 84 kDa
    • ab81290, at 1/50, staining human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    References for Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290)

    ab81290 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"