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Rabbit polyclonal to RSPH3
antigen sequence corresponding to amino acids 268-354 (IIEVDMECQT DAFLDRPPTP LFIPAKTGKD VATQILEGEL FDFDLEVKPV LEVLVGKTIE QSLLEVMEEE ELANLRASQR EYEELRN) of Human RSPH3, isoform 1.
- Human testis tissue; RT-4 and U-251 MG cell lysates; Human Plasma, Liver and Tonsil lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Belongs to the flagellar radial spoke RSP3 family.
Information by UniProt
- dJ111C20.1 antibody
- Radial spoke 3 homolog (Chlamydomonas) antibody
- Radial spoke head 3 homolog antibody
Immunocytochemistry/ Immunofluorescence - Anti-RSPH3 antibody (ab122535)
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122535 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPH3 antibody (ab122535)
ab122535, at 1/250 dilution, staining RSPH3 in paraffin-embedded Human testis tissue by Immunohistochemistry, showing moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Western blot - Anti-RSPH3 antibody (ab122535)
All lanes : Anti-RSPH3 antibody (ab122535) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Plasma lysate
Lane 5 : Human Liver lysate
Lane 6 : Human Tonsil lysate
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"