Overview

  • Product name
    Anti-RSPH3 antibody
  • Description
    Rabbit polyclonal to RSPH3
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence corresponding to amino acids 268-354 (IIEVDMECQT DAFLDRPPTP LFIPAKTGKD VATQILEGEL FDFDLEVKPV LEVLVGKTIE QSLLEVMEEE ELANLRASQR EYEELRN) of Human RSPH3, isoform 1.

  • Positive control
    • Human testis tissue; RT-4 and U-251 MG cell lysates; Human Plasma, Liver and Tonsil lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Belongs to the flagellar radial spoke RSP3 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ111C20.1 antibody
    • Radial spoke 3 homolog (Chlamydomonas) antibody
    • Radial spoke head 3 homolog antibody
    • Radial spoke head protein 3 homolog antibody
    • Radial spoke head-like protein 2 antibody
    • Radial spoke protein 3 antibody
    • Radial spokehead like 2 antibody
    • RSHL2 antibody
    • RSP3 antibody
    • RSPH3 antibody
    • RSPH3_HUMAN antibody
    see all

Anti-RSPH3 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122535 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122535, at 1/250 dilution, staining RSPH3 in paraffin-embedded Human testis tissue by Immunohistochemistry, showing moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
  • All lanes : Anti-RSPH3 antibody (ab122535) at 1/250 dilution

    Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human Liver lysate
    Lane 6 : Human Tonsil lysate

References for Anti-RSPH3 antibody (ab122535)

ab122535 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122535.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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