Overview

  • Product nameAnti-RSPH4A antibody
    See all RSPH4A primary antibodies
  • Description
    Rabbit polyclonal to RSPH4A
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 98-164 (APARQDLAAP PQSDRTTSVI PEAGTPYPDP LEQSSDKRES TPHHTSQSEG NTFQQSQQPK PHLCGRR) of Human RSPH4A.

  • Positive control
    • Human bronchus tissue; A-431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab122158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionProbable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules.
  • Involvement in diseaseDefects in RSPH4A are the cause of primary ciliary dyskinesia type 11 (CILD11) [MIM:612649]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
  • Sequence similaritiesBelongs to the flagellar radial spoke RSP4/6 family.
  • Cellular localizationCytoplasm > cytoskeleton > cilium axoneme. Radial spoke.
  • Information by UniProt
  • Database links
  • Alternative names
    • A230081C05 antibody
    • CILD11 antibody
    • dJ412I7.1 antibody
    • Radial spoke head protein 4 homolog A antibody
    • Radial spoke head-like protein 3 antibody
    • RSH4A_HUMAN antibody
    • RSHL3 antibody
    • Rsph4a antibody
    • RSPH6B antibody
    see all

Anti-RSPH4A antibody images

  • ab122158, at 1/75, staining RSPH4A in paraffin embedded Human bronchus by Immunohistochemistry.
  • ab122158, at 4 µg/ml, staining RSPH4A (green) in PFA-fixed, Triton-permeabilized Human A-431 cells by Immunofluorescence.

References for Anti-RSPH4A antibody (ab122158)

ab122158 has not yet been referenced specifically in any publications.

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