Anti-RSPH4A antibody (ab122159)
Key features and details
- Rabbit polyclonal to RSPH4A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RSPH4A antibody
See all RSPH4A primary antibodies -
Description
Rabbit polyclonal to RSPH4A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: DVSYNNAKQK ELRFDVFQEE DSNSDYDLQQ PAPGGSEVAP SMLEITIQNA KAYLLKTSSN SGFNLYDHLS NMLTKI, corresponding to amino acids 165-240 of Human RSPH4A.
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Positive control
- Human kidney tissue; RT4 and U51MG lysates; A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/250 - 1/500.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
Target
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Function
Probable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules. -
Involvement in disease
Defects in RSPH4A are the cause of primary ciliary dyskinesia type 11 (CILD11) [MIM:612649]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. -
Sequence similarities
Belongs to the flagellar radial spoke RSP4/6 family. -
Cellular localization
Cytoplasm > cytoskeleton > cilium axoneme. Radial spoke. - Information by UniProt
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Database links
- Entrez Gene: 345895 Human
- Omim: 612647 Human
- SwissProt: Q5TD94 Human
- Unigene: 160380 Human
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Alternative names
- A230081C05 antibody
- CILD11 antibody
- dJ412I7.1 antibody
see all
Images
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ab122159 at 4 µg/ml staining RSPH4A in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence(green).
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ab122159 at 1/100 dilution staining RSPH4A in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
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All lanes : Anti-RSPH4A antibody (ab122159) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251MG lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
Immunohistochemical staining of Human fallopian tube shows strong positivity in ciliated cells using ab122159
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122159 has not yet been referenced specifically in any publications.