Overview

  • Product nameAnti-RSPH4A antibody
    See all RSPH4A primary antibodies
  • Description
    Rabbit polyclonal to RSPH4A
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: DVSYNNAKQK ELRFDVFQEE DSNSDYDLQQ PAPGGSEVAP SMLEITIQNA KAYLLKTSSN SGFNLYDHLS NMLTKI, corresponding to amino acids 165-240 of Human RSPH4A.

  • Positive control
    • Human kidney tissue; RT4 and U51MG lysates; A431 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Using PFA/Triton X-100 fixation
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • FunctionProbable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules.
  • Involvement in diseaseDefects in RSPH4A are the cause of primary ciliary dyskinesia type 11 (CILD11) [MIM:612649]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
  • Sequence similaritiesBelongs to the flagellar radial spoke RSP4/6 family.
  • Cellular localizationCytoplasm > cytoskeleton > cilium axoneme. Radial spoke.
  • Information by UniProt
  • Database links
  • Alternative names
    • A230081C05 antibody
    • CILD11 antibody
    • dJ412I7.1 antibody
    • Radial spoke head protein 4 homolog A antibody
    • Radial spoke head-like protein 3 antibody
    • RSH4A_HUMAN antibody
    • RSHL3 antibody
    • Rsph4a antibody
    • RSPH6B antibody
    see all

Anti-RSPH4A antibody images

  • ab122159 at 1/100 dilution staining RSPH4A in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • All lanes : Anti-RSPH4A antibody (ab122159) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U251MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab122159 at 4 µg/ml staining RSPH4A in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence(green).
  • Immunohistochemical staining of Human fallopian tube shows strong positivity in ciliated cells using ab122159

References for Anti-RSPH4A antibody (ab122159)

ab122159 has not yet been referenced specifically in any publications.

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