Anti-RSPO1 antibody [7A6] (ab81600)

Overview

  • Product nameAnti-RSPO1 antibody [7A6]See all RSPO1 primary antibodies ...
  • Description
    Mouse monoclonal [7A6] to RSPO1
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of human RSPO1 expressed in E.coli.

  • Positive control
    • Recombinant RSPO1; RSPO1 (aa21-263)-hIgGFc transfected HEK293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/2000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA 1/10000.

Target

  • FunctionActivator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.
  • Tissue specificityAbundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
  • Involvement in diseaseDefects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.
  • Sequence similaritiesBelongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • DomainThe FU repeats are required for activation and stabilization of beta-catenin.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession Q2MKA7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CRISTIN3 antibody
    • FLJ40906 antibody
    • hRspo1 antibody
    • R spondin homolog (Xenopus laevis) antibody
    • R spondin homolog antibody
    • R spondin1 antibody
    • R-spondin homolog (Xenopus laevis) antibody
    • R-spondin-1 antibody
    • R-spondin1 antibody
    • Roof plate specific spondin antibody
    • roof plate-specific spondin antibody
    • Roof plate-specific spondin-1 antibody
    • RP11-566C13.1 antibody
    • RSPO antibody
    • Rspo1 antibody
    • RSPO1_HUMAN antibody
    see all

Anti-RSPO1 antibody [7A6] images

  • All lanes : Anti-RSPO1 antibody [7A6] (ab81600) at 1/500 dilution

    Lane 1 : Recombinant RSPO1
    Lane 2 : RSPO1 (aa21-263)-hIgGFc transfected HEK293 cell lysate


    Predicted band size : 29 kDa
    Observed band size : 29,70 kDa (why is the actual band size different from the predicted?)

References for Anti-RSPO1 antibody [7A6] (ab81600)

ab81600 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I wish to apologize for the delay in attaining information about this immunogen for you. I have been in contact with the lab on your behalf.Unfortunately, we do not have the information of the amino acid range or...

Read More
Application Western blot
Sample Human Recombinant protein (Recombinant protein expressed in Hek293)
Loading amount 40000 cells
Specification Recombinant protein expressed in Hek293
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jan 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"