Overview

  • Product name
    Anti-RSPO1 antibody
  • Description
    Rabbit polyclonal to RSPO1
  • Specificity
    At least three isoforms of RSPO1 are known to exist; ab106556 will detect the largest and smallest isoforms. ab106556 will not cross-react with other RSPO family members.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human RSPO1 (N terminal).
    Database link: Q2MKA7

  • Positive control
    • Rat spleen tissue lysate; Human spleen tissue, Human heart tissue lysate
  • General notes

    Antibodies should not be exposed to prolonged high temperatures.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with RSPO1 peptide (ab214172).
IHC-P Use a concentration of 2.5 - 20 µg/ml.

Target

  • Function
    Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.
  • Tissue specificity
    Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
  • Involvement in disease
    Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.
  • Sequence similarities
    Belongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • Domain
    The FU repeats are required for activation and stabilization of beta-catenin.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CRISTIN3 antibody
    • FLJ40906 antibody
    • hRspo1 antibody
    • R spondin homolog (Xenopus laevis) antibody
    • R spondin homolog antibody
    • R spondin1 antibody
    • R-spondin-1 antibody
    • Roof plate specific spondin antibody
    • Roof plate-specific spondin-1 antibody
    • RP11-566C13.1 antibody
    • RSPO antibody
    • Rspo1 antibody
    • RSPO1_HUMAN antibody
    see all

Anti-RSPO1 antibody images

  • Lane 1 : Anti-RSPO1 antibody (ab106556) at 1 µg/ml
    Lane 2 : Anti-RSPO1 antibody (ab106556) at 2 µg/ml

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human heart tissue lysate


    Predicted band size : 29 kDa
  • Immunohistochemical analysis of PFA fixed paraffin embedded Human spleen tissue sections labelling RSPO1 with ab106556, at 2.5 µg/mL. 

  • Lane 1 : Anti-RSPO1 antibody (ab106556) at 1 µg/ml
    Lane 2 : Anti-RSPO1 antibody (ab106556) at 2 µg/ml

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 29 kDa
  • Immunohistochemical analysis of Human spleen tissue labelling RSPO1 with ab106556 at 20 ug/mL.

     

  • Immunohistochemical analysis of PFA fixed paraffin embedded Human heart tissue sections labelling RSPO1 with ab106556, at 2.5 µg/mL. 

References for Anti-RSPO1 antibody (ab106556)

This product has been referenced in:
  • Shi GX  et al. Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. Int J Mol Sci 18:N/A (2017). WB ; Mouse . Read more (PubMed: 28272338) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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