The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notesIs unsuitable for WB.
RelevanceRSPO2 is part of the R-spondin family of proteins that consist of secreted cysteine-rich proteins that can activate beta-catenin signaling via the Frizzled/LRP5/6 receptor complex. R-spondin 2 is required for normal laryngeal-tracheal, lung and limb morphogenesis. RSPO2 expression in required in the apical ectodermal ridge for normal hindlimb development.