The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
RelevanceRSPO2 is part of the R-spondin family of proteins that consist of secreted cysteine-rich proteins that can activate beta-catenin signaling via the Frizzled/LRP5/6 receptor complex. R-spondin 2 is required for normal laryngeal-tracheal, lung and limb morphogenesis. RSPO2 expression in required in the apical ectodermal ridge for normal hindlimb development.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RSPO2 antibody (ab73761)Image courtesy of an anonymous Abreview.
ab73761 staining RSPO2 in human meniscus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed using Protease24. Samples were then blocked using 1% BSA for 15 minutes at 21°C, then incubated with ab73761 at a 1/100 dilution for 16 hours at 21°C. The secondary used was an undiluted biotin conjugated goat anti-rabbit polyclonal.