Overview

  • Product nameAnti-RSPO2 antibody
    See all RSPO2 primary antibodies
  • Description
    Rabbit polyclonal to RSPO2
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RSPO2.

    (Peptide available as ab98902.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: LOVO; SW480; Caco 2; HCT 116

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab73761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).

Target

  • RelevanceRSPO2 is part of the R-spondin family of proteins that consist of secreted cysteine-rich proteins that can activate beta-catenin signaling via the Frizzled/LRP5/6 receptor complex. R-spondin 2 is required for normal laryngeal-tracheal, lung and limb morphogenesis. RSPO2 expression in required in the apical ectodermal ridge for normal hindlimb development.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • CRISTIN2 antibody
    • hRspo2 antibody
    • R spondin 2 antibody
    • R spondin 2 homolog antibody
    • R-spondin 2 homolog (Xenopus laevis) antibody
    • R-spondin family, member 2 antibody
    • Roof plate-specific spondin-2 antibody
    see all

Anti-RSPO2 antibody images

  • All lanes : Anti-RSPO2 antibody (ab73761) at 1 µg/ml

    Lane 1 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 106 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ab73761 staining RSPO2 in human meniscus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed using Protease24. Samples were then blocked using 1% BSA for 15 minutes at 21°C, then incubated with ab73761 at a 1/100 dilution for 16 hours at 21°C. The secondary used was an undiluted biotin conjugated goat anti-rabbit polyclonal.

    See Abreview

References for Anti-RSPO2 antibody (ab73761)

ab73761 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (meniscus)
Loading amount 200 µg
Specification meniscus
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Aug 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (meniscus)
Specification meniscus
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Protease24
Permeabilization No
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"