Anti-RSPO3 antibody (ab109808)


  • Product nameAnti-RSPO3 antibody
    See all RSPO3 primary antibodies
  • Description
    Goat polyclonal to RSPO3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 181-193 of Human RSPO3 (NP_116173.2). RSPO3(Peptide available as ab135941.)

  • Positive control
    • Human skeletal muscle lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab109808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human RSPO3 peptide (ab135941).


  • FunctionActivator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.
  • Tissue specificityUbiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node.
  • Sequence similaritiesBelongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • DomainThe FU repeats are required for activation and stabilization of beta-catenin.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CRISTIN1 antibody
    • hPWTSR antibody
    • hRspo3 antibody
    • Protein with TSP type-1 repeat antibody
    • PWTSR antibody
    • R-spondin 3 homolog antibody
    • R-spondin-3 [Precursor] antibody
    • R-spondin-3 antibody
    • Roof plate specific spondin-3 antibody
    • Roof plate-specific spondin-3 antibody
    • RSPO3 antibody
    • RSPO3_HUMAN antibody
    • Thrombospondin type I domain containing 2 antibody
    • Thrombospondin type-1 domain-containing protein 2 antibody
    • THSD2 antibody
    see all

Anti-RSPO3 antibody images

  • Anti-RSPO3 antibody (ab109808) at 0.3 µg/ml + Human skeletal muscle lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 hour

References for Anti-RSPO3 antibody (ab109808)

ab109808 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse embryo section)
Specification mouse embryo section
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 15 2013

I would like to inform you that we have been able to place the RSPO3 peptide used to create ab190808 on to our catalog. The product number for this is ab135945. The datasheet for this product may be found at the following address: http://www.abcam.c...

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Thank you for contacting us.

I have contacted the lab to get this peptide added to our catalog. These may take a few days but I will let you know when it has been added.

I hope this information is helpful to you. Please do not...

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