Anti-RSPO3 antibody (ab229726)
Key features and details
- Rabbit polyclonal to RSPO3
- Suitable for: WB
- Reacts with: Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-RSPO3 antibody
See all RSPO3 primary antibodies -
Description
Rabbit polyclonal to RSPO3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant fragment within Zebrafish RSPO3 (internal sequence). The exact sequence is proprietary.
Database link: Q5R328 -
Positive control
- WB: Zebrafish brain and ovary tissue extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
Target
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Function
Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. -
Tissue specificity
Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node. -
Sequence similarities
Belongs to the R-spondin family.
Contains 2 FU (furin-like) repeats.
Contains 1 TSP type-1 domain. -
Domain
The FU repeats are required for activation and stabilization of beta-catenin. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 100007702 Zebrafish
- SwissProt: Q5R328 Zebrafish
- Unigene: 75336 Zebrafish
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Alternative names
- CRISTIN1 antibody
- hPWTSR antibody
- hRspo3 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229726 has not yet been referenced specifically in any publications.