Recombinant
RabMAb

Anti-RSPO4 antibody [EPR9806(2)] (ab189515)

Overview

  • Product name
    Anti-RSPO4 antibody [EPR9806(2)]
    See all RSPO4 primary antibodies
  • Description
    Rabbit monoclonal [EPR9806(2)] to RSPO4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RSPO4 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q2I0M5

  • Positive control
    • Mouse and rat brain, C6, Raw 264.7, NIH3T3 cell lysates; Human RSPO4 recombinant protein.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR9806(2)
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab189515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 26 kDa.

Target

  • Function
    Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors.
  • Involvement in disease
    Defects in RSPO4 are the cause of anonychia congenita (ANONC) [MIM:206800]. A rare condition characterized by the absence or severe hypoplasia of all fingernails and toenails without significant bone anomalies.
  • Sequence similarities
    Belongs to the R-spondin family.
    Contains 1 FU (furin-like) repeat.
    Contains 1 TSP type-1 domain.
  • Domain
    The FU repeat is required for activation and stabilization of beta-catenin.
  • Post-translational
    modifications
    Tyr-112 may be phosphorylated; however as this position is probably extracellular, the vivo relevance is not proven.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20orf182 antibody
    • CRISTIN4 antibody
    • hRspo4 antibody
    • R-spondin family, member 4 antibody
    • R-spondin-4 antibody
    • Roof plate-specific spondin-4 antibody
    • RSPO4 antibody
    • RSPO4_HUMAN antibody
    see all

Images

  • Anti-RSPO4 antibody [EPR9806(2)] (ab189515) at 1/20000 dilution + Human RSPO4 recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 26 kDa
  • All lanes : Anti-RSPO4 antibody [EPR9806(2)] (ab189515) at 1/5000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Rat brain
    Lane 3 : C6
    Lane 4 : Raw 264.7 cell lysate
    Lane 5 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000

    Predicted band size : 26 kDa

References

ab189515 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189515.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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