Overview

  • Product name
  • Description
    Rabbit polyclonal to RSU1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within Synthetic peptide: GVSHVFEYIR SETYKYLYGR HMQANPEPPK KNNDKSKKIS RKPLAAKNR, corresponding to C terminal amino acids 229-277 of Human RSU1

  • Positive control
    • Jurkat cell lysate and Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab69843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Images

  • ab69843 at 8µg/ml staining RSU1 in epithelial cells of human kidney renal tubule (indicated by arrows) by Immunohistochemistry, Paraffin-embedded tissue.
  • Anti-RSU1 antibody (ab69843) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Donthamsetty S  et al. Role of PINCH and Its Partner Tumor Suppressor Rsu-1 in Regulating Liver Size and Tumorigenesis. PLoS One 8:e74625 (2013). Read more (PubMed: 24058607) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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