Overview

  • Product nameAnti-RT1-Aw2 antibody [MRC OX-18]
    See all RT1-Aw2 primary antibodies
  • Description
    Mouse monoclonal [MRC OX-18] to RT1-Aw2
  • Specificityab119782 recognizes a monomorphic determinant of rat Class I MHC antigen (RT1.A) and thus reacts with all rat strains tested including AO(RT1µ), DA (RT1a), LEW (RT11) and PVG (RT1c).
  • Tested applicationsSuitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat spleen membrane glycoproteins depleted of Ia-A antigens.

  • Positive control
    • Thymus (38.1% cells stained); Spleen (97.0% cells stained); Lymph Node (99.9% cells stained).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from ascitic fluid
  • ClonalityMonoclonal
  • Clone numberMRC OX-18
  • Myelomax63-Ag8.653
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2-0.5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration.

Target

  • FunctionInvolved in the presentation of foreign antigens to the immune system.
  • Sequence similaritiesBelongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Class I histocompatibility antigen antibody
    • HA11_RAT antibody
    • MHCRTA antibody
    • Non-RT1.A alpha-1 chain antibody
    • RATMHCRTA antibody
    • RT1 A antibody
    • RT1-Aw2 antibody
    • RT1.EC2 antibody
    • RT1A antibody
    • RTS antibody
    see all

Anti-RT1-Aw2 antibody [MRC OX-18] images

  • Rat Strain: Wistar
    Cell Concentration: 1x106 cells
    Antibody Concentration Used: 0.2 µg/106 cells
    Isotypic Control: Mouse IgG
    Cell Source: Spleen
    Percentage of cells stained: 97.0% .

References for Anti-RT1-Aw2 antibody [MRC OX-18] (ab119782)

This product has been referenced in:
  • Fukumoto T  et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 (1982). Read more (PubMed: 6178598) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Rat Tissue lysate - whole (Hypothalamus)
Specification Hypothalamus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (hippocampus)
Loading amount 20 µg
Specification hippocampus
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Jana Aradska

Verified customer

Submitted Aug 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"