Anti-RT1-Ba antibody [MRC OX-3] (Phycoerythrin) (ab123125)

Overview

  • Product nameAnti-RT1-Ba antibody [MRC OX-3] (Phycoerythrin)
    See all RT1-Ba primary antibodies
  • Description
    Mouse monoclonal [MRC OX-3] to RT1-Ba (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab123125 recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, ACI, DA, or PVG/c strains. This antibody also cross-reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9. ab123125 recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes. Thus, ab123125 is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Rat thymocyte membrane glycoprotein

  • Positive control
    • Rat Strain: Buffalo (Thymus, Spleen, Lymph Node)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberMRC OX-3
  • MyelomaP3-NS1/1-Ag4-1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.05µg for 106 cells.

Target

  • Cellular localizationIntegral to membrane
  • Database links
  • Alternative names
    • MHC class II antigen RT1.B alpha chain antibody
    • Rano class II histocompatibility antigen, B alpha chain antibody
    • RT1 class II antigen, Ba chain antibody
    • RT1 class II, locus Ba antibody

Anti-RT1-Ba antibody [MRC OX-3] (Phycoerythrin) images

  • Rat Strain: Buffalo
    Isotypic Control: PE Mouse IgG1. Cell Source: Spleen.
    Percentage of cells stained above control: 43.9%

References for Anti-RT1-Ba antibody [MRC OX-3] (Phycoerythrin) (ab123125)

This product has been referenced in:
  • Barclay AN Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens. Immunology 44:727-36 (1981). Read more (PubMed: 6119291) »
  • Barclay AN & Mayrhofer G Bone marrow origin of Ia-positive cells in the medulla rat thymus. J Exp Med 153:1666-71 (1981). Read more (PubMed: 6942092) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab123125.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"