Overview

  • Product nameAnti-RTCD1 antibody
    See all RTCD1 primary antibodies
  • Description
    Mouse polyclonal to RTCD1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    RTCD1 full-length Human protein (NP_003720)

  • Positive control
    • Human spleen lysate and RTCD1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.

Target

  • RelevanceRTCD1 catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. There are two named isoforms.
  • Cellular localizationNucleus, nucleoplasm.
  • Database links
  • Alternative names
    • 2310009A18Rik antibody
    • AI450277 antibody
    • EC 6.5.1.4 antibody
    • MGC102342 antibody
    • MGC94866 antibody
    • RNA 3' terminal phosphate cyclase antibody
    • RNA 3-prime-terminal phosphate cyclase antibody
    • RNA cyclase antibody
    • RNA terminal phosphate cyclase domain 1 antibody
    • RNA terminal phosphate cyclase domain containing protein 1 antibody
    • RNA-3'-phosphate cyclase antibody
    • RPC antibody
    • RPC1 antibody
    • RTC domain containing protein 1 antibody
    • RTC1 antibody
    • RTCA antibody
    • RTCD1 antibody
    see all

Anti-RTCD1 antibody images

  • Anti-RTCD1 antibody (ab89043) at 1 µg/ml + Human spleen lysate at 50 µg

    Predicted band size : 39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RTCD1 antibody (ab89043) at 1 µg/ml

    Lane 1 : RTCD1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa

References for Anti-RTCD1 antibody (ab89043)

ab89043 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"