Overview

  • Product nameAnti-RTCD1 antibody
    See all RTCD1 primary antibodies
  • Description
    Rabbit polyclonal to RTCD1
  • SpecificityDetects endogenous levels of total RTCD1 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from an internal sequence of Human RTCD1

  • Positive control
    • Jurkat cell lysate, Human breast carcinoma tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: 1/5000.
    IHC-P: 1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
    WB: 1/500 - 1/1000. Predicted molecular weight: 39 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceRTCD1 catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. There are two named isoforms.
    • Cellular localizationNucleus, nucleoplasm.
    • Database links
    • Alternative names
      • 2310009A18Rik antibody
      • AI450277 antibody
      • EC 6.5.1.4 antibody
      • MGC102342 antibody
      • MGC94866 antibody
      • RNA 3' terminal phosphate cyclase antibody
      • RNA 3-prime-terminal phosphate cyclase antibody
      • RNA cyclase antibody
      • RNA terminal phosphate cyclase domain 1 antibody
      • RNA terminal phosphate cyclase domain containing protein 1 antibody
      • RNA-3'-phosphate cyclase antibody
      • RPC antibody
      • RPC1 antibody
      • RTC domain containing protein 1 antibody
      • RTC1 antibody
      • RTCA antibody
      • RTCD1 antibody
      see all

    Anti-RTCD1 antibody images

    • All lanes : Anti-RTCD1 antibody (ab92661) at 1/500 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cell lysate with blocking peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 39 kDa
    • ab92661 at 1/50 dilution staining RTCD1 in (1) Human breast carcinoma and (2) Human breast carcinoma in presence of a blocking peptide.

    References for Anti-RTCD1 antibody (ab92661)

    ab92661 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"