Overview

  • Product name
  • Description
    Rabbit polyclonal to RTF1
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
  • Immunogen

    Immunogen corresponding to C terminal amino acids within Human RTF1 (EAW92492.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab99362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA.
  • Sequence similarities
    Contains 1 Plus3 domain.
  • Domain
    The Plus3 domain mediates single-stranded DNA-binding.
  • Cellular localization
    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDG1N antibody
    • GTL 7 antibody
    • GTL7 antibody
    • KIAA0252 antibody
    • Ortholog of mouse gene trap locus 7 antibody
    • RNA polymerase associated protein RTF1 homolog antibody
    • RNA polymerase-associated protein RTF1 homolog antibody
    • RTF 1 antibody
    • RTF1 antibody
    • Rtf1 Paf1/RNA polymerase II complex component homolog antibody
    • RTF1_HUMAN antibody
    • rtf1L antibody
    • YGL244W antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and mouse teratoma (right) tissues labelling RTF1 with ab99362 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-RTF1 antibody (ab99362) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 77 kDa


    Exposure time : 10 seconds
  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99362 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: RTF1 antibody (ab99362) at 1 µg/ml

    developed using the ECL technique

    Exposure time : 3 seconds

    Predicted band size : 77 kDa

References

This product has been referenced in:
  • Eirin A  et al. Comparative proteomic analysis of extracellular vesicles isolated from porcine adipose tissue-derived mesenchymal stem/stromal cells. Sci Rep 6:36120 (2016). Read more (PubMed: 27786293) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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