Overview

  • Product name
  • Description
    Mouse polyclonal to RTN3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    RTN3 (NP_006045.1, 1 a.a. ~ 236 a.a) full length human protein.

  • Positive control
    • RTN3 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa for isoform 3).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis.
    • Tissue specificity
      Isoform 3 is widely expressed, with highest levels in brain, where it is enriched in neuronal cell bodies from gray matter (at protein level). Three times more abundant in macula than in peripheral retina. Isoform 1 is expressed at high levels in brain and at low levels in skeletal muscle. Isoform 2 is only found in melanoma.
    • Sequence similarities
      Contains 1 reticulon domain.
    • Cellular localization
      Endoplasmic reticulum membrane. Golgi apparatus membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ASYIP antibody
      • Neuroendocrine specific protein like 2 antibody
      • Neuroendocrine-specific protein-like 2 antibody
      • Neuroendocrine-specific protein-like II antibody
      • NSP like protein II antibody
      • NSP-like protein 2 antibody
      • NSP-like protein II antibody
      • NSPL2 antibody
      • NSPLII antibody
      • Reticulon 3 antibody
      • Reticulon-3 antibody
      • Reticulon3 antibody
      • Rtn3 antibody
      • RTN3_HUMAN antibody
      see all

    Images

    • All lanes : Anti-RTN3 antibody (ab68328) at 1/500 dilution

      Lane 1 : RTN3 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 26 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Li B  et al. Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma. Sci Rep 7:5517 (2017). WB . Read more (PubMed: 28717245) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hela)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    Hela
    Blocking step
    Fish Skin Gelatin (FSG) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 05 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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