Anti-Rubella Virus capsid antibody [9B11] (ab34749)

Overview

  • Product name
    Anti-Rubella Virus capsid antibody [9B11]
  • Description
    Mouse monoclonal [9B11] to Rubella Virus capsid
  • Specificity
    This antibody does not react with measles and mumps virus nucleocapsid proteins.
  • Tested applications
    Suitable for: Indirect ELISA, WBmore details
  • Species reactivity
    Reacts with rubella. Not yet tested in other species.
  • Immunogen

    Recombinant full length protein corresponding to Rubella Virus capsid.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    9B11
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000. Predicted molecular weight: 115 kDa.

Target

  • Relevance
    Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It causes German measles ( a mild contagious eruptive disease, capable of producing congenital defects in infants born to mothers infected during the first three months of pregnancy). Rubella virus is an enveloped positive-strand RNA virus. The genome encodes two open reading frames (ORFs): the 5'-proximal ORF encodes viral nonstructural proteins (NSP) that are responsible for viral genome replication, while the 3'-proximal ORF encodes three virion structural proteins (SP), the capsid protein (CP), and the two envelope glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase.
  • Cellular localization
    Cytoplasmic in host cells concentrated around Golgi region and mitochondrion.
  • Alternative names
    • Coat protein antibody
    • p110 antibody
    • rubella capsid antibody
    • Structural polyprotein antibody

Images

  • All lanes : Anti-Rubella Virus capsid antibody [9B11] (ab34749) at 1 µg/ml

    Lane 1 : Prestained M.Wt markers
    Lane 2 : crude yeast cell lysate [mock control]
    Lane 3 : irrelevant viral proteins
    Lane 4 : irrelevant viral proteins
    Lane 5 : recombinant purified rubella capsid protein
    Lane 6 : crude lysate of transformed yeast cells expressing rubella capsid protein


    Predicted band size : 115 kDa

References

This product has been referenced in:
  • Perelygina L  et al. Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies. J Allergy Clin Immunol 138:1436-1439.e11 (2016). IF . Read more (PubMed: 27613149) »
  • Perelygina L  et al. Differences in Establishment of Persistence of Vaccine and Wild Type Rubella Viruses in Fetal Endothelial Cells. PLoS One 10:e0133267 (2015). Read more (PubMed: 26177032) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BlockAid as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
Sample
Human Tissue sections (Lung)
Specification
Lung
Permeabilization
Yes - 0.2% Triton X-100
Fixative
Formaldehyde
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Verified customer

Submitted Apr 10 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Human Cell (HUVEC)
Specification
HUVEC
Permeabilization
No
Fixative
Methanol
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Verified customer

Submitted Apr 10 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% PAGE)
Sample
African Green Monkey Cell lysate - whole cell (Vero cells)
Specification
Vero cells
Treatment
infected with rubella virus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Apr 10 2015

Thank you for contacting us.  I am still waiting for additional information regarding the strain used to generate ab106292, but I will let you know as soon as I hear back from the lab regarding this.  The other products are as follows: ab34749: 9B11 wa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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