Overview

  • Product name
  • Description
    Rabbit polyclonal to Rubicon
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, from a region within amino acids 922-972 of Human Rubicon (NP_055502.1)

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab92388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-Fr Use at an assay dependent concentration. PubMed: 22843690

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Baron antibody
    • Beclin 1 associated RUN domain containing protein antibody
    • Beclin-1 associated RUN domain containing protein antibody
    • hypothetical protein LOC9711 antibody
    • Kiaa0226 antibody
    • OTTHUMP00000208021 antibody
    • OTTHUMP00000208023 antibody
    • OTTHUMP00000208024 antibody
    • RUBIC_HUMAN antibody
    • Rubicon antibody
    • RUN domain and cysteine rich domain containing, Beclin 1 interacting protein antibody
    • Run domain Beclin 1 interacting and cystein rich containing protein antibody
    • Run domain Beclin-1 interacting and cysteine-rich containing protein antibody
    • RUN domain protein as Beclin 1 interacting and cysteine rich containing antibody
    see all

Images

  • All lanes : Anti-Rubicon antibody (ab92388) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 109 kDa


    Exposure time : 3 minutes
  • Sample: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
    Antibodies: ab92388 used for WB 1 µg/ml (lane 1) and used for IP at 3 µg/mg lysate. Lane 2: control IgG.
    Detection: chemiluminescence with exposure time of 30 seconds.
  • ICC/IF image of ab92388 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92388, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of Human renal cancer tissue, staining Rubicon with ab92388. Tissue was fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.
  • Immunohistochemical analysis of Human renal tumour cells, staining Rubicon with ab92388. Cells were fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.

References

This product has been referenced in:
  • Sun T  et al. Acetylation of Beclin 1 inhibits autophagosome maturation and promotes tumour growth. Nat Commun 6:7215 (2015). Read more (PubMed: 26008601) »
  • Bruntz RC  et al. Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells. J Biol Chem 289:600-16 (2014). WB ; Human . Read more (PubMed: 24257753) »

See all 3 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Microglia/astrocyte)
Permeabilization
Yes - 0.3% Troton X-100
Specification
Microglia/astrocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Apr 11 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kiney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kiney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Jun 20 2016

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kiney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kiney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jun 02 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

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Verified customer

Submitted Jun 02 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% triton x-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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