Anti-Rubicon antibody - N-terminal (ab173930)

Overview

  • Product name
    Anti-Rubicon antibody - N-terminal
    See all Rubicon primary antibodies
  • Description
    Rabbit polyclonal to Rubicon - N-terminal
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Rubicon aa 249-278 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q92622

  • Positive control
    • MCF7 cell lysate; U251 cells; Human lymph tissue; MDA-231 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab173930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 109 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Baron antibody
    • Beclin 1 associated RUN domain containing protein antibody
    • Beclin-1 associated RUN domain containing protein antibody
    • hypothetical protein LOC9711 antibody
    • Kiaa0226 antibody
    • OTTHUMP00000208021 antibody
    • OTTHUMP00000208023 antibody
    • OTTHUMP00000208024 antibody
    • RUBIC_HUMAN antibody
    • Rubicon antibody
    • RUN domain and cysteine rich domain containing, Beclin 1 interacting protein antibody
    • Run domain Beclin 1 interacting and cystein rich containing protein antibody
    • Run domain Beclin-1 interacting and cysteine-rich containing protein antibody
    • RUN domain protein as Beclin 1 interacting and cysteine rich containing antibody
    see all

Images

  • Anti-Rubicon antibody - N-terminal (ab173930) at 1/50 dilution + MCF7 cell lysate at 35 µg

    Predicted band size : 109 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lymph tissue labeling Rubicon with ab173930 at 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Flow cytometric analysis of MDA-231 cells labeling Rubicon with ab173930 at 1/10 dilution (bottom histogram), compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunofluorescent analysis of U251 cells labeling Rubicon with ab173930 at 1/100 dilution. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ab173930 (1/100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

References

ab173930 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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