Overview

  • Product name
  • Description
    Chicken polyclonal to RUBISCO
  • Host species
    Chicken
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Spinacia oleracea
    Predicted to work with: Plants
  • Immunogen

    Full length protein; RUBISCO small subunit (Spinach). NCBI Acc : AAC29137.1, Swiss-Prot Q9TIM3.

  • Positive control
    • Purified spinach RUBISCO.

Properties

Applications

Our Abpromise guarantee covers the use of ab62391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 15, 45 kDa (predicted molecular weight: 55 kDa).

Target

  • Function
    RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
  • Sequence similarities
    Belongs to the RuBisCO large chain family. Type I subfamily.
  • Post-translational
    modifications
    The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.
  • Cellular localization
    Plastid > chloroplast.
  • Information by UniProt
  • Database links
    • Alternative names
      • 3 phospho D glycerate carboxylase antibody
      • rbcL antibody
      • RBL_SPIOL antibody
      • Ribulose 1,5 bisphosphate carboxylase/oxygenase small subunit N methyltransferase I antibody
      • Ribulose bisphosphate carboxylase large chain antibody
      • RuBisCO large subunit antibody
      see all

    Images

    • Anti-RUBISCO antibody (ab62391) at 1/2000 dilution + Purified spinach RUBISCO protein at 1 µg

      Secondary
      Goat anti-IgY-HRP at 1/1000 dilution

      Predicted band size: 55 kDa
      Observed band size: 15,45 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Cai Z  et al. Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco. Protein Cell 5:552-62 (2014). WB . Read more (PubMed: 24870149) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for contacting us with your questions. We have only tested these antibodies under reducing conditions in WB. They may be suitable for use under non-reducing conditions as well, but this has not been validated in-house. I hope this information...

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    Thank you for contacting us.  We have only tested these Peroxiredoxin antibodies under reducing conditions in WB.  They may be suitable for use under non-reducing conditions as well, but this has not been validated in house.  The unconjugated secondary...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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