Overview

  • Product nameAnti-RUBISCO antibody
  • Description
    Chicken polyclonal to RUBISCO
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Spinacia oleracea
    Predicted to work with: Plants
  • Immunogen

    Full length protein; RUBISCO small subunit (Spinach). NCBI Acc : AAC29137.1, Swiss-Prot Q9TIM3.

  • Positive control
    • Purified spinach RUBISCO.

Properties

Applications

Our Abpromise guarantee covers the use of ab62391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/2000. Detects bands of approximately 15 and 45 kDa (predicted molecular weight of precursor: 55 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
    • Sequence similaritiesBelongs to the RuBisCO large chain family. Type I subfamily.
    • Post-translational
      modifications
      The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.
    • Cellular localizationPlastid > chloroplast.
    • Information by UniProt
    • Database links
      • Alternative names
        • 3 phospho D glycerate carboxylase antibody
        • rbcL antibody
        • RBL_SPIOL antibody
        • Ribulose 1,5 bisphosphate carboxylase/oxygenase small subunit N methyltransferase I antibody
        • Ribulose bisphosphate carboxylase large chain antibody
        • RuBisCO large subunit antibody
        see all

      Anti-RUBISCO antibody images

      • Anti-RUBISCO antibody (ab62391) at 1/2000 dilution + Purified spinach RUBISCO protein at 1 µg

        Secondary
        Goat anti-IgY-HRP at 1/1000 dilution

        Predicted band size : 55 kDa
        Observed band size : 15,45 kDa (why is the actual band size different from the predicted?)

      References for Anti-RUBISCO antibody (ab62391)

      This product has been referenced in:
      • Cai Z  et al. Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco. Protein Cell 5:552-62 (2014). WB . Read more (PubMed: 24870149) »

      See 1 Publication for this product

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"