Overview

  • Product name
  • Description
    Rabbit polyclonal to RUFY1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 293-464 of Human RUFY1 (AAH32571).

  • Positive control
    • U87-MG whole cell lysate; IMR32 and MCF7 cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: Isoform 1, 80 ; Isoform 2, 69 kDa.

Target

  • Function
    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
  • Tissue specificity
    Broadly expressed, with highest levels in lung, testis, kidney and brain.
  • Sequence similarities
    Contains 1 FYVE-type zinc finger.
    Contains 1 RUN domain.
  • Domain
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Post-translational
    modifications
    Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3000002E04Rik antibody
    • FLJ22251 antibody
    • FYVE finger protein EIP1 antibody
    • FYVE-finger protein EIP1 antibody
    • La binding protein 1 antibody
    • La-binding protein 1 antibody
    • OTTHUMP00000161521 antibody
    • Rab4 interacting protein antibody
    • Rab4-interacting protein antibody
    • RABIP4 antibody
    • RUFY1 antibody
    • RUFY1_HUMAN antibody
    • RUN and FYVE domain containing 1 antibody
    • RUN and FYVE domain-containing protein 1 antibody
    • RUN and FYVE domains containing protein 1 antibody
    • ZFYVE12 antibody
    • Zinc finger FYVE domain containing protein 12 antibody
    • Zinc finger FYVE domain-containing protein 12 antibody
    see all

Images

  • Anti-RUFY1 antibody (ab101519) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size : Isoform 1, 80 ; Isoform 2, 69 kDa

References

ab101519 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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