Overview

  • Product nameAnti-RUFY1 antibody
    See all RUFY1 primary antibodies
  • Description
    Goat polyclonal to RUFY1
  • Specificityab109804 is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical proteins: NP_001035542.1 and NP_001035541.1.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    C-QKNEAITSFEGKT

    , corresponding to internal sequence amino acids 479-491 of Human RUFY1 (NP_079434.3; NP_001035541.1).

  • Positive control
    • HeLa lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab109804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).

Target

  • FunctionBinds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
  • Tissue specificityBroadly expressed, with highest levels in lung, testis, kidney and brain.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
    Contains 1 RUN domain.
  • DomainThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Post-translational
    modifications
    Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3000002E04Rik antibody
    • FLJ22251 antibody
    • FYVE finger protein EIP1 antibody
    • FYVE-finger protein EIP1 antibody
    • La binding protein 1 antibody
    • La-binding protein 1 antibody
    • OTTHUMP00000161521 antibody
    • Rab4 interacting protein antibody
    • Rab4-interacting protein antibody
    • RABIP4 antibody
    • RUFY1 antibody
    • RUFY1_HUMAN antibody
    • RUN and FYVE domain containing 1 antibody
    • RUN and FYVE domain-containing protein 1 antibody
    • RUN and FYVE domains containing protein 1 antibody
    • ZFYVE12 antibody
    • Zinc finger FYVE domain containing protein 12 antibody
    • Zinc finger FYVE domain-containing protein 12 antibody
    see all

Anti-RUFY1 antibody images

  • Anti-RUFY1 antibody (ab109804) at 0.05 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 80 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References for Anti-RUFY1 antibody (ab109804)

ab109804 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"