Overview

  • Product nameAnti-RUFY4 antibody
    See all RUFY4 primary antibodies
  • Description
    Mouse polyclonal to RUFY4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human RUFY4.

  • Positive control
    • Human liver and FLJ46536 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detected bands of approximately 38 kDa and 46 kDa in two separate blots (predicted molecular weight for isoform 2 of this protein: 45 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-RUFY4 antibody images

    • Anti-RUFY4 antibody (ab69388) at 1/500 dilution + Human liver at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 45 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-RUFY4 antibody (ab69388) at 1/500 dilution

      Lane 1 : FLJ46536 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 45 kDa
      Observed band size : 46 kDa (why is the actual band size different from the predicted?)

    References for Anti-RUFY4 antibody (ab69388)

    ab69388 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab69388.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"