Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980)

Western blot - RUNX1 / AML1 antibody - ChIP Grade (ab23980)

All lanes : Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) at 1/1000 dilution

Lane 1 : Mouse Bladder
Lane 2 : Mouse Lung
Lane 3 : Mouse Liver
Lane 4 : Mouse Thymus

Secondary
Donkey polyclonal to anti-Rabbit IgG, HRP-Linked F(ab')2 Fragment at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 48 kDa

Megan Crow, King's college London, United Kingdom

Western blot - Runx1/AML1 antibody (ab23980)

Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844) at 20 µg

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution

Predicted band size : 48 kDa
Observed band size : 48,52,55 kDa (why is the actual band size different from the predicted?)


This antibody recognized three distinct bands of between 48 and 55 kDa in Jurkat nuclear lysate. These may represent distinct isoforms of Runx1 or may represent post-translationally modified forms.

ChIP - RUNX1 / AML1 antibody - ChIP Grade (ab23980)

Chromatin was prepared from nuclear lysate of the mouse haematopoietic progenitor cell line HPC-7. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 10 minutes in 1% formaldehyde. The primary antibody was used undiluted and incubated with the sample for 16 hours at 4ºC. The immunoprecipitated DNA was quantified by real time PCR. Negative control: A regultory element which is an open region of chromatin but is devoid of this transcription factor.

This image is a courtesy of Anonymous Abreview

Immunocytochemistry/ Immunofluorescence - RUNX1 / AML1 antibody - ChIP Grade (ab23980)

ab23980 staining RUNX1 / AML1 in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4ºC. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.

Image courtesy of an anonymous Abreview.