Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980)

Overview

  • Product nameAnti-RUNX1 / AML1 antibody - ChIP Grade
    See all RUNX1 / AML1 primary antibodies
  • Description
    Rabbit polyclonal to RUNX1 / AML1 - ChIP Grade
  • SpecificityAlthough some customers are able to use this antibody to cross react with mouse samples, we are unable to reproduce this consistently. Therefore this product is no longer guaranteed for use in this species.
  • Tested applicationsSuitable for: ChIP, EMSA, WB, CHIPseq, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Runx1/AML1.

    (Peptide available as ab24287.)

  • Positive control
    • Jurkat Nuclear Lysate
  • General notes

    Antibody batches of a concentration <1mg/ml will have BSA added to them.

Properties

Applications

Our Abpromise guarantee covers the use of ab23980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration. PubMed: 22253448
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
CHIPseq Use at an assay dependent concentration. PubMed: 24760698
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 21151104

Target

  • FunctionCBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
  • Tissue specificityExpressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
  • Involvement in diseaseNote=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
    Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
    Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
    Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
    Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16.
  • Sequence similaritiesContains 1 Runt domain.
  • DomainA proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
  • Post-translational
    modifications
    Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
    Methylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acute myeloid leukemia 1 antibody
    • Acute myeloid leukemia 1 protein antibody
    • alpha subunit core binding factor antibody
    • AML 1 antibody
    • AML1 antibody
    • AML1 EVI 1 antibody
    • AML1 EVI 1 fusion protein antibody
    • Aml1 oncogene antibody
    • AMLCR 1 antibody
    • AMLCR1 antibody
    • CBF alpha 2 antibody
    • CBF-alpha-2 antibody
    • CBFA 2 antibody
    • CBFA2 antibody
    • Core binding factor alpha 2 subunit antibody
    • Core binding factor runt domain alpha subunit 2 antibody
    • Core-binding factor subunit alpha-2 antibody
    • EVI 1 antibody
    • EVI1 antibody
    • HGNC antibody
    • Oncogene AML 1 antibody
    • Oncogene AML-1 antibody
    • OTTHUMP00000108696 antibody
    • OTTHUMP00000108697 antibody
    • OTTHUMP00000108699 antibody
    • OTTHUMP00000108700 antibody
    • OTTHUMP00000108702 antibody
    • PEA2 alpha B antibody
    • PEA2-alpha B antibody
    • PEBP2 alpha B antibody
    • PEBP2-alpha B antibody
    • PEBP2A2 antibody
    • PEBP2aB antibody
    • Polyomavirus enhancer binding protein 2 alpha B subunit antibody
    • Polyomavirus enhancer-binding protein 2 alpha B subunit antibody
    • Run1 antibody
    • Runt related transcription factor 1 antibody
    • Runt-related transcription factor 1 antibody
    • RUNX 1 antibody
    • Runx1 antibody
    • RUNX1_HUMAN antibody
    • SL3 3 enhancer factor 1 alpha B subunit antibody
    • SL3-3 enhancer factor 1 alpha B subunit antibody
    • SL3/AKV core binding factor alpha B subunit antibody
    • SL3/AKV core-binding factor alpha B subunit antibody
    see all

Anti-RUNX1 / AML1 antibody - ChIP Grade images

  • Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) at 1 µg/ml + Jurkat nuclear extract lysate (ab14844) at 20 µg

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48,52,55 kDa (why is the actual band size different from the predicted?)
    This antibody recognized three distinct bands of between 48 and 55 kDa in Jurkat nuclear lysate. These may represent distinct isoforms of Runx1 or may represent post-translationally modified forms.
  • ab23980 staining RUNX1 / AML1 in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to FITC used at a 1/400 dilution. Nuclei are counterstained with DAPI

    See Abreview

  • ab23980 staining RUNX1 / AML1 in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.

References for Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980)

This product has been referenced in:
  • Aini H  et al. Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment. Sci Rep 6:18743 (2016). IHC-P ; Mouse . Read more (PubMed: 26728350) »
  • Zheng S  et al. Twist1 and Twist2 Contribute to Cytokine Downregulation following Chronic NOD2 Stimulation of Human Macrophages through the Coordinated Regulation of Transcriptional Repressors and Activators. J Immunol 195:217-26 (2015). ChIP ; Human . Read more (PubMed: 26019273) »

See all 23 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Thymus)
Gel Running Conditions Reduced Denaturing (gel 12%)
Loading amount 10 µg
Specification Thymus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (macrophage)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (macrophags)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 1e+006 cells
Specification macrophags
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 09 2014

Hello!

The immunogen sequence has only 70% homology with RUNX2 and no significant similarity with RUNX3 and is very unlikely to cross-react. Usually around 85% there is cross-reactivity. Please let me know if I can be of further assistance.

Thank you for contacting us. The actual immunogen is a smaller peptide within that sequence. The tag is for purification purposes but it is not necessary that your samples have a tag for Runx1 to be detected. This antibody has been tested for use in mo...

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Thank you for contacting Abcam.

As we discussed, I am sending you a Jurkat cell lysate to use as a positive control with ab23980 to check if the antibody is working correctly. You should receive this lysate on Friday and your order number is *...

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Thank you for contacting Abcam earlier today.

I received your voicemail about the lot number that your colleague was sent. We are out of stock of that particular lot number, but if you have any issues whatsoever with the vial that we are send...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

To check the status of the order please contact our Customer Service team and reference this numb...

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Thank you for bringing this to our attention. I think your protocol with the dilutions you are using should not be giving you high background, especially if the myc western blot demonstrates that the samples are not degraded.

We have a new l...

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Merci beaucoup pour ces précisions.

N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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