Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-RUNX1 / AML1 antibody - ChIP Grade
See all RUNX1 / AML1 products (14) ...
Description
Rabbit polyclonal to RUNX1 / AML1 - ChIP Grade
Tested applications
ChIP, WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Runx1/AML1.
(Peptide available as ab24287.)
Positive control
Jurkat Nuclear Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Hematopoietic system development
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Hematopoietic Progenitors >> Hemangioblast
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution. (PubMed: 21151104)
Target
Function
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
Tissue specificity
Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
Involvement in disease
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16.
Sequence similarities
Contains 1 Runt domain.
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
Post-translational
modifications
Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
Methylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ01196
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Acute myeloid leukemia 1 antibody
- Acute myeloid leukemia 1 protein antibody
- alpha subunit antibody
see all
Database links
- Entrez Gene: 861 Human
- Entrez Gene: 12394 Mouse
- Entrez Gene: 50662 Rat
- Omim: 151385 Human
- SwissProt: Q01196 Human
- SwissProt: Q03347 Mouse
- SwissProt: Q63046 Rat
- Unigene: 149261 Human
see all
Anti-RUNX1 / AML1 antibody - ChIP Grade images:
Western blot - RUNX1 / AML1 antibody - ChIP Grade (ab23980)
All lanes : Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) at 1/1000 dilution
Lane 1 : Mouse Bladder
Lane 2 : Mouse Lung
Lane 3 : Mouse Liver
Lane 4 : Mouse Thymus
Secondary
Donkey polyclonal to anti-Rabbit IgG, HRP-Linked F(ab')2 Fragment at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 48 kDa
Megan Crow, King's college London, United Kingdom
Western blot - Runx1/AML1 antibody (ab23980)
Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution
Predicted band size : 48 kDa
Observed band size : 48,52,55 kDa (why is the actual band size different from the predicted?)
This antibody recognized three distinct bands of between 48 and 55 kDa in Jurkat nuclear lysate. These may represent distinct isoforms of Runx1 or may represent post-translationally modified forms.
ChIP - RUNX1 / AML1 antibody - ChIP Grade (ab23980)
Chromatin was prepared from nuclear lysate of the mouse haematopoietic progenitor cell line HPC-7. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 10 minutes in 1% formaldehyde. The primary antibody was used undiluted and incubated with the sample for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR. Negative control: A regultory element which is an open region of chromatin but is devoid of this transcription factor.
This image is a courtesy of Anonymous Abreview
Immunocytochemistry/ Immunofluorescence - RUNX1 / AML1 antibody - ChIP Grade (ab23980)
ab23980 staining RUNX1 / AML1 in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-RUNX1 / AML1 antibody - ChIP Grade (ab23980)
This product has been referenced in:
- Lazarevic Vet al. T-bet represses T(H)17 differentiation by preventing Runx1-mediated activation of the gene encoding ROR?t. Nat Immunol 12:96-104 (2011). WB, IP; Human.Read more (PubMed: 21151104) »
- Farrell JJet al. A 3-bp deletion in the HBS1L-MYB intergenic region on chromosome 6q23 is associated with HbF expression. Blood 117:4935-45 (2011).Read more (PubMed: 21385855) »
See all 6 publications for this product
Publishing research using ab23980? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"