Synthetic peptide corresponding to residues at the C-terminus of Human RUNX1/ AML1.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab92336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Predicted molecular weight: 49 kDa.Can be blocked with RUNX1 / AML1 peptide (ab177141).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody will give a stronger signal.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
RUNX2 recombinant protein full length, with N-terminal HIS tag, expressed in E.Coli.
RUNX3 overexpression and empty vector control lysates created in HEK293T cells. The protein contains a C-terminal DDK tag.