Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336)

Overview

Applications

Our Abpromise guarantee covers the use of ab92336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 49 kDa.Can be blocked with RUNX1 / AML1 peptide (ab177141).
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody will give a stronger signal.

Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

Target

Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] images


  • Developed using the ECL technique

    Predicted band size : 49 kDa


    Exposure time : 1 minute

    RUNX2 recombinant protein full length, with N-terminal HIS tag, expressed in E.Coli.

    RUNX3 overexpression and empty vector control lysates created in HEK293T cells.  The protein contains a C-terminal DDK tag.

  • All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1/10000 dilution

    Lane 1 : MOLT4 cell lysate
    Lane 2 : fetal thymus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 49 kDa
  • Immunohistochemistry staining of RUNX1 / AML1 in formalin-fixed, paraffin-embedded Human tonsil tissue using 1/100 ab92336.
  • Flow cytometric analysis of permeabilized Molt-4 cells using anti-RUNX1 ab92336 (red) or a rabbit IgG (negative) (green).
  • ab92336 staining RUNX1 / AML1 in human glioblastoma cell line by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

  • ab92336 staining RUNX1 / AML1 in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

References for Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336)

This product has been referenced in:
  • Ponder KL  et al. Preeclampsia and Inflammatory Preterm Labor Alter the Human Placental Hematopoietic Niche. Reprod Sci 23:1179-92 (2016). Read more (PubMed: 26944948) »
  • Treanor LM  et al. Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential. J Exp Med 211:701-13 (2014). Read more (PubMed: 24687960) »

See all 9 Publications for this product

Product Wall

Merci de nous avoir contactés.

J'ai donc comme convenu vérifié les séquences immunogènes et les alignements de séquences (voir fichiers joints)pour les deux monoclonaux de lapin anti-RUNX1 / AML1:
...

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Merci pour votre appel.

J'ai fait l'alignement de l-ab92336 avec la séquence de poulet, mais c'est pas super. Veuillez voir vous-même: http://www.ebi.ac.uk/Tools/services/web_clustalo/toolresult.ebi?tool=clustalo&jobId=clusta...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line U87MG)
Specification human glioblastoma cell line U87MG
Fixative Paraformaldehyde
Permeabilization Yes - 1% Triton X 100 in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioblastoma cell line from rat)
Specification glioblastoma cell line from rat
Fixative Paraformaldehyde
Permeabilization Yes - 1% Triton X 100 in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioblastoma cell line C6)
Specification glioblastoma cell line C6
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 24 2012

Thank you for bringing this to our attention.  I have forwarded your data to our lab for their input.  We may end up submitting a recall for this product, but I would like for them to review your results.  I have refunded your purchase. I am sorry...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"