Anti-RUNX1T1 / ETO antibody (ab124269)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-RUNX1T1 / ETO antibody
See all RUNX1T1 / ETO primary antibodies -
DescriptionRabbit polyclonal to RUNX1T1 / ETO
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 554-604 of Human RUNX1T1/ ETO (NP_001185555.1).
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Positive control
- Purchase matching WB positive control:Recombinant Human RUNX1T1 / ETO protein
- 293T whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7 to 8 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab124269 was affinity purified using an epitope specific to RUNX1T1 / ETO immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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FunctionTranscription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12.
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Tissue specificityMost abundantly expressed in brain. Lower levels in lung, heart, testis and ovary.
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Involvement in diseaseNote=A chromosomal aberration involving RUNX1T1 is a cause of acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1/AML1.
Defects in RUNX1T1 may be a cause of colorectal cancer (CRC) [MIM:114500]. -
Sequence similaritiesBelongs to the CBFA2T family.
Contains 1 MYND-type zinc finger.
Contains 1 TAFH (NHR1) domain. -
DomainThe TAFH domain mediates interaction with transcription regulators.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 862 Human
- Entrez Gene: 12395 Mouse
- Entrez Gene: 362489 Rat
- Omim: 133435 Human
- SwissProt: Q06455 Human
- SwissProt: Q61909 Mouse
- Unigene: 368431 Human
- Unigene: 470961 Mouse
see all -
Alternative names
- Acute myelogenous leukemia 1 translocation 1 cyclin D related antibody
- Acute myelogenous leukemia 1 translocation 1 protein antibody
- AML1 MTG8 antibody
see all
Images
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Western blot - Anti-RUNX1T1 / ETO antibody (ab124269)All lanes : Anti-RUNX1T1 / ETO antibody (ab124269) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds -
Immunoprecipitation - Anti-RUNX1T1 / ETO antibody (ab124269)ab124269 at 1 µg/ml staining RUNX1T1 / ETO in 293T whole cell lysate immunoprecipitated using ab124269 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 68 kDa. Exposure time: 30 seconds
References
This product has been referenced in:
- Zaidi SK et al. An AML1-ETO/miR-29b-1 regulatory circuit modulates phenotypic properties of acute myeloid leukemia cells. Oncotarget 8:39994-40005 (2017). Read more (PubMed: 28611288) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"