The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000.
Use at 2-10 µg/mg of lysate.
Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12.
Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary.
Involvement in disease
Note=A chromosomal aberration involving RUNX1T1 is a cause of acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1/AML1. Defects in RUNX1T1 may be a cause of colorectal cancer (CRC) [MIM:114500].
Belongs to the CBFA2T family. Contains 1 MYND-type zinc finger. Contains 1 TAFH (NHR1) domain.
The TAFH domain mediates interaction with transcription regulators.
Acute myelogenous leukemia 1 translocation 1 cyclin D related antibody
Acute myelogenous leukemia 1 translocation 1 protein antibody
AML1 MTG8 antibody
Core binding factor runt domain alpha subunit 2 translocated to 1 antibody
Core binding factor runt domain alpha subunit 2 translocated to 1 cyclin D related antibody
Cyclin D related antibody
Cyclin D related protein antibody
Cyclin-D-related protein antibody
Eight twenty one protein antibody
ETO protein antibody
MTG 8 antibody
MTG 8b antibody
MTG8 protein antibody
Myeloid translocation gene on 8q22 antibody
Protein CBFA2T1 antibody
Protein ETO antibody
Protein MTG 8 antibody
Protein MTG8 antibody
Runt related transcription factor 1 translocated to 1 (cyclin D related) antibody
Runt related transcription factor 1 translocated to 1 antibody
Runt related transcription factor 1 translocated to 1 cyclin D related antibody
RUNX1 translocation partner 1 antibody
Zinc finger MYND domain containing protein 2 antibody
Zinc finger MYND domain-containing protein 2 antibody
ZMYND 2 antibody
Western blot - Anti-RUNX1T1 / ETO antibody (ab124269)
All lanes : Anti-RUNX1T1 / ETO antibody (ab124269) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 50 µg Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique
Exposure time : 30 seconds
Immunoprecipitation - Anti-RUNX1T1 / ETO antibody (ab124269)
ab124269 at 1 µg/ml staining RUNX1T1 / ETO in 293T whole cell lysate immunoprecipitated using ab124269 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 68 kDa.
Exposure time: 30 seconds