Anti-RUNX1T1 / ETO antibody (ab26161)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab26161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12.
  • Tissue specificityMost abundantly expressed in brain. Lower levels in lung, heart, testis and ovary.
  • Involvement in diseaseNote=A chromosomal aberration involving RUNX1T1 is a cause of acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1/AML1.
    Defects in RUNX1T1 may be a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similaritiesBelongs to the CBFA2T family.
    Contains 1 MYND-type zinc finger.
    Contains 1 TAFH (NHR1) domain.
  • DomainThe TAFH domain mediates interaction with transcription regulators.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acute myelogenous leukemia 1 translocation 1 cyclin D related antibody
    • Acute myelogenous leukemia 1 translocation 1 protein antibody
    • AML1T1 antibody
    • CBFA2T1 antibody
    • CDR antibody
    • Core binding factor runt domain alpha subunit 2 translocated to 1 antibody
    • Core binding factor runt domain alpha subunit 2 translocated to 1 cyclin D related antibody
    • Cyclin D related antibody
    • Cyclin D related protein antibody
    • Cyclin-D-related protein antibody
    • Eight twenty one protein antibody
    • ETO antibody
    • ETO protein antibody
    • MGC2796 antibody
    • MTG 8 antibody
    • MTG 8b antibody
    • MTG8 antibody
    • MTG8 protein antibody
    • MTG8_HUMAN antibody
    • MTG8b antibody
    • Myeloid translocation gene on 8q22 antibody
    • Protein CBFA2T1 antibody
    • Protein ETO antibody
    • Protein MTG 8 antibody
    • Protein MTG8 antibody
    • Runt related transcription factor 1 translocated to 1 (cyclin D related) antibody
    • Runt related transcription factor 1 translocated to 1 antibody
    • Runt related transcription factor 1 translocated to 1 cyclin D related antibody
    • RUNX1T1 antibody
    • Zinc finger MYND domain containing protein 2 antibody
    • Zinc finger MYND domain-containing protein 2 antibody
    • ZMYND 2 antibody
    • ZMYND2 antibody
    see all

Anti-RUNX1T1 / ETO antibody images

  • Anti-RUNX1T1 / ETO antibody (ab26161) at 1.25 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50000 - 100000

    Predicted band size : 62 kDa

References for Anti-RUNX1T1 / ETO antibody (ab26161)

ab26161 has not yet been referenced specifically in any publications.

Product Wall

RUNX1T1/ETO has two splice variants: http://www.uniprot.org/uniprot/Q06455. Moreover, the weak lower band could be due to posttranslational modifications- eg different phosphorylation state.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"