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Overview
Product name
Anti-RUNX2 antibody
See all RUNX2 products (12) ...
Description
Mouse monoclonal to RUNX2
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Recombinant fragment: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA , corresponding to amino acids 251-351 of Human RUNX2
NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 57 kDa.
IHC-P: Use a concentration of 3 µg/ml
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation.
Tissue specificity
Specifically expressed in osteoblasts.
Involvement in disease
Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.
Sequence similarities
Contains 1 Runt domain.
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites.
Post-translational
modifications
Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ13950
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- PEBP2aA antibody
- Acute myeloid leukemia 3 protein antibody
- Alpha subunit 1 antibody
see all
Database links
- Entrez Gene: 860 Human
- Entrez Gene: 367218 Rat
- Omim: 600211 Human
- SwissProt: Q13950 Human
- SwissProt: Q9Z2J9 Rat
- Unigene: 535845 Human
- Unigene: 214214 Rat
- Unigene: 83672 Rat
Anti-RUNX2 antibody images:
Western blot - RUNX2 antibody (ab54868)
All lanes : Anti-RUNX2 antibody (ab54868) at 1/1000 dilution
Lane 1 : UMR-106 Rat osteosarcoma cell line, nuclear cell lysate
Lane 2 : UMR-106 Rat osteosarcoma cell line, cytoplasmic cell lysate
Secondary
HRP conjugated goat anti-mouse antibody at 1/500 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57/59,65,98 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
The blot was blocked with 1% milk for 1 hour at RT and incubated with the primary antibody for 1 hour.
This image is courtesy of an Abreview submitted by Dr Cesar Camacho
Western blot - RUNX2 antibody (ab54868)
Anti-RUNX2 antibody (ab54868) at 1 µg/ml + Cell lysate prepared from K-562 cells
Predicted band size : 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RUNX2 antibody (ab54868)
ab54868 at 3 ug/ml staining RUNX2 in human placenta tissue section by Immunohistochemistry (Formalin /PFA fixed paraffin embedded tissue sections).
References for Anti-RUNX2 antibody (ab54868)
This product has been referenced in:
- Custódio MRet al. Parathyroid hormone and phosphorus overload in uremia: impact on cardiovascular system. Nephrol Dial Transplant : (2011).Read more (PubMed: 21825304) »
- Graciolli FGet al. Phosphorus overload and PTH induce aortic expression of Runx2 in experimental uraemia. Nephrol Dial Transplant 24:1416-21 (2009).Read more (PubMed: 19075196) »
See all 2 publications for this product
Publishing research using ab54868? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"