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Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab192256 as a replacement.
Our Abpromise guarantee covers the use of ab76956 in the following tested applications.
|ELISA||Use at an assay dependent concentration. Detection limit for recombinant tagged RUNX2 is approximately 0.1ng/ml when used as a capture antibody.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 21159735|
|Flow Cyt||Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.|
ab76956 staining RUNX2 in Mouse dveloping endochondral bone tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton and blocked with 10% Roche Western Blocking for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in blocking solution) for 16 hours. An undiluted Alexa Fluor® 555-conjugated Donkey polyclonal was used as the secondary antibody.