• Product nameAnti-RUNX3 antibody
    See all RUNX3 primary antibodies
  • Description
    Rabbit polyclonal to RUNX3
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cat, Dog
  • Immunogen

    A region within synthetic peptide: GAPQSQSGPF QANPAPYHLF YGASSGSYQF SMAAAGGGER SPTRMLTSCP, corresponding to C terminal amino acids 326-375 of Mouse RUNX3

  • Positive control
    • NIH3T3 cell lysates


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab49117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/12500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration. PubMed: 21205319


  • FunctionCBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
  • Sequence similaritiesContains 1 Runt domain.
  • DomainA proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
  • Post-translational
    Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
  • Cellular localizationNucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acute myeloid leukemia 2 protein antibody
    • Acute myeloid leukemia gene 2 antibody
    • AML 2 antibody
    • AML2 antibody
    • CBF alpha 3 antibody
    • CBF-alpha-3 antibody
    • CBFA 3 antibody
    • CBFA3 antibody
    • Core binding factor alpha 3 subunit antibody
    • core binding factor antibody
    • Core binding factor runt domain alpha subunit 3 antibody
    • Core binding factor subunit alpha 3 antibody
    • core-binding factor antibody
    • Core-binding factor subunit alpha-3 antibody
    • Oncogene AML 2 antibody
    • Oncogene AML-2 antibody
    • PEA2 alpha C antibody
    • PEA2-alpha C antibody
    • PEBP2 alpha C antibody
    • PEBP2-alpha C antibody
    • Pebp2a3 antibody
    • PEBP2aC antibody
    • Polyomavirus enhancer binding protein 2 alpha C subunit antibody
    • Polyomavirus enhancer-binding protein 2 alpha C subunit antibody
    • runt domain alpha subunit 3 antibody
    • runt related transcription factor 3 antibody
    • Runt-related transcription factor 3 antibody
    • RUNX 3 antibody
    • Runx3 antibody
    • RUNX3_HUMAN antibody
    • SL3 3 enhancer factor 1 alpha C subunit antibody
    • SL3-3 enhancer factor 1 alpha C subunit antibody
    • SL3/AKV core binding factor alpha C subunit antibody
    • SL3/AKV core-binding factor alpha C subunit antibody
    • Transcription factor AML2 antibody
    see all

Anti-RUNX3 antibody images

  • Anti-RUNX3 antibody (ab49117) at 1.25 µg/ml + NIH3T3 cell lysate at 10 µg

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

References for Anti-RUNX3 antibody (ab49117)

This product has been referenced in:
  • Wang Z  et al. Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes. J Cell Mol Med 18:1807-15 (2014). WB . Read more (PubMed: 24889513) »
  • Nakanishi Y  et al. Loss of runt-related transcription factor 3 expression leads hepatocellular carcinoma cells to escape apoptosis. BMC Cancer 11:3 (2011). WB, IHC-P ; Human . Read more (PubMed: 21205319) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 120 µg
Gel Running Conditions Reduced Denaturing (12,5)
Sample Zebrafish Tissue lysate - whole (whole embryo lysate)
Specification whole embryo lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 25 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human osteosarcoma)
Specification Human osteosarcoma
Fixative Paraformaldehyde
Permeabilization Yes - PBS TRITON 0.25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 11 2012

Thank you for your reply.

Below please find the recipe for 1mM EDTA, adjusted to pH 8.0:

EDTA 0.37 g
Distilled water 1000 ml
Store at room temperature for 3 months.

Tris would not be used to pH ...

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Thank you for your inquiry.

For ab49117, we would guarantee this antibody to work in IHC-P in mouse, however we have not tested this in house. We know that IHC-P has been tested with human samples in this reference:

Nakanishi Y et a...

Read More