The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight of Isoform d: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceRUSC1 is a putative signaling adapter which may play a role in neuronal differentiation and may be involved in regulation of NGF-dependent neurite outgrowth. It seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK.
Cellular localizationCytoplasm. Nucleus. Note=Translocated to the nuclear envelope upon stimulation with NGF.