Overview

  • Product nameAnti-RuvB antibody
    See all RuvB primary antibodies
  • Description
    Rabbit polyclonal to RuvB
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Full length native protein (purified) (E. coli).

Properties

Applications

Our Abpromise guarantee covers the use of ab18581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 37 kDa.
IP Use at an assay dependent dilution.

Target

  • RelevanceIn Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC,RuvAB is uncoupled from RuvC and DSBs may be prevented.
  • Cellular localizationCell Membrane, Cytoplasmic and Nuclear
  • Database links
    • Alternative names
      • ATP dependent DNA helicase, component of RuvABC resolvasome antibody
      • Holliday junction ATP dependent DNA helicase ruvB antibody
      • Holliday junction helicase subunit A antibody
      • RUVB (recombination protein) homolog antibody

    References for Anti-RuvB antibody (ab18581)

    This product has been referenced in:
    • Lim B  et al. Heat shock transcription factor s(32) co-opts the signal recognition particle to regulate protein homeostasis in E. coli. PLoS Biol 11:e1001735 (2013). WB ; Escherichia coli . Read more (PubMed: 24358019) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"