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Anti-RWDD1 antibody [EPR13715(B)]
Rabbit monoclonal [EPR13715(B)] to RWDD1
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RWDD1 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q9H446
HeLa cells; Jurkat, HeLa, 293, A673 cell lysate
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Protects DRG2 from proteolytic degradation.
Belongs to the RWDD1/GIR2 family.
Contains 1 RWD domain.
Information by UniProt
CGI 24 antibody
DRG family regulatory protein 2 antibody
Western blot - Anti-RWDD1 antibody [EPR13715(B)] (ab181994)
All lanes : Anti-RWDD1 antibody [EPR13715(B)] (ab181994) at 1/50000 dilution Lane 1 : Jurkat cell lysate Lane 2 : Hela cell lysate Lane 3 : 293 cell lysate Lane 4 : A673 cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution Predicted band size: 28 kDa
Flow Cytometry - Anti-RWDD1 antibody [EPR13715(B)] (ab181994)
Flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling RWDD1 with ab181994 at a 1/10 dilution, or with rabbit IgG isotype control. Goat anti rabbit IgG (FITC) at a 1/150 dilution was used as a secondary antibody.
Immunoprecipitation - Anti-RWDD1 antibody [EPR13715(B)] (ab181994)
Immunoprecipitation of Jurkat labeling RWDD1 using ab181994 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"