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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RWDD1 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q9H446
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab181994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).|
|Flow Cyt||1/10. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
Flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling RWDD1 with ab181994 at a 1/10 dilution, or with rabbit IgG isotype control. Goat anti rabbit IgG (FITC) at a 1/150 dilution was used as a secondary antibody.
Immunoprecipitation of Jurkat labeling RWDD1 using ab181994 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST
ab181994 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"