Overview

  • Product nameAnti-RWDD3 antibody
    See all RWDD3 primary antibodies
  • Description
    Rabbit polyclonal to RWDD3
  • SpecificityAt least four isoforms of RWDD3 are known to exist; this antibody will only detect the largest isoform.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid synthetic peptide from near the C-terminus of Human RWDD3 (NP_056300)

  • Positive control
    • Rat kidney tissue lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab91555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 31 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEnhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination.
    • Tissue specificityExpressed in a wide number of tissues with highest expression in cerebellum, pituitary, heart, kidney, liver, stomach, pancreas, prostate and spleen. Low levels in thalamus, spinal cord, esophagus, thymus, lung and peripheral blood leukocytes.
    • Sequence similaritiesContains 1 RWD domain.
    • DomainThe RWD domain is required for the sumoylation enhancement activity.
    • Cellular localizationNucleus. Cytoplasm. Colocalizes with UBC9/UBE2I in nuclear spots.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp566K023 antibody
      • RSUME antibody
      • RWD containing sumoylation enhancer antibody
      • RWD domain containing 3 antibody
      • RWD domain containing protein antibody
      • RWD domain containing protein 3 antibody
      • RWD domain-containing protein 3 antibody
      • RWD-containing sumoylation enhancer antibody
      • rwdd3 antibody
      • RWDD3_HUMAN antibody
      see all

    Anti-RWDD3 antibody images

    • All lanes : Anti-RWDD3 antibody (ab91555) at 1 µg/ml

      Lane 1 : Rat kidney tissue lysate
      Lane 2 : Rat kidney tissue lysate with blocking peptide

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 31 kDa

    References for Anti-RWDD3 antibody (ab91555)

    ab91555 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your call this week and for your patience while I have looked into your enquiry. The blocking peptide for ab91555 is now available as ab117773- http://www.abcam.com/RWDD3-peptide-ab117773.html Please let me know if yo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"