Overview

  • Product name
  • Description
    Rabbit polyclonal to RWDD4A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 139 - 188 (SKKKDKKEQL SKAQKRKLAD KTDHKGELPR GWNWVDVVKH LSKTGSKDDE) of Human RWDD4A (NP_689895)

  • Positive control
    • SH-SYSY cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-RWDD4A antibody images

  • Anti-RWDD4A antibody (ab90065) at 1 µg/ml + SH-SYSY cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa

References for Anti-RWDD4A antibody (ab90065)

ab90065 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
120 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Apr 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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