Anti-Ryanodine receptor 2/RYR-2 antibody - N-terminal (ab196355)
Key features and details
- Rabbit polyclonal to Ryanodine receptor 2/RYR-2 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ryanodine receptor 2/RYR-2 antibody - N-terminal
See all Ryanodine receptor 2/RYR-2 primary antibodies -
Description
Rabbit polyclonal to Ryanodine receptor 2/RYR-2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit -
Immunogen
Synthetic peptide within Human Ryanodine receptor 2/RYR-2 (N terminal). The exact sequence is proprietary.
Database link: Q92736 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 1.21% Tris, 0.75% Glycine -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development. -
Tissue specificity
Detected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta. -
Involvement in disease
Familial arrhythmogenic right ventricular dysplasia 2
Ventricular tachycardia, catecholaminergic polymorphic 1, with or without atrial dysfunction and/or dilated cardiomyopathy -
Sequence similarities
Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR2 subfamily.
Contains 3 B30.2/SPRY domains.
Contains 5 MIR domains. -
Developmental stage
Expressed in myometrium during pregnancy. -
Domain
The calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm. -
Post-translational
modificationsChannel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels.
Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium. -
Cellular localization
Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 6262 Human
- Entrez Gene: 20191 Mouse
- Entrez Gene: 100009536 Rabbit
- Entrez Gene: 689560 Rat
- Omim: 180902 Human
- SwissProt: Q92736 Human
- SwissProt: E9Q401 Mouse
- SwissProt: P30957 Rabbit
see all -
Alternative names
- ARVC2 antibody
- ARVD2 antibody
- Cardiac muscle ryanodine receptor antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab196355 has been referenced in 3 publications.
- Qian D et al. Trans-cinnamaldehyde protects against phenylephrine-induced cardiomyocyte hypertrophy through the CaMKII/ERK pathway. BMC Complement Med Ther 22:115 (2022). PubMed: 35468773
- Zhang X et al. STK25 inhibits PKA signaling by phosphorylating PRKAR1A. Cell Rep 40:111203 (2022). PubMed: 35977512
- Ruiz-Meana M et al. Ryanodine Receptor Glycation Favors Mitochondrial Damage in the Senescent Heart. Circulation 139:949-964 (2019). PubMed: 30586718