Anti-Ryanodine receptor 2 (phospho S2808) antibody (ab59225)

Overview

  • Product nameAnti-Ryanodine receptor 2 (phospho S2808) antibody
    See all Ryanodine receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to Ryanodine receptor 2 (phospho S2808)
  • Specificityab59225 detects endogenous levels of Ryanodine Receptor 2 only when phosphorylated at serine 2808.
  • Tested applicationsSuitable for: IHC-P, ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Ryanodine receptor 2. Synthetic phosphopeptide derived from human Ryanodine Receptor around the phosphorylation site of serine 2808 (R-I-SP-Q-T).
    Database link: Q92736

  • Positive control
    • Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/5000.
ICC/IF Use at an assay dependent concentration. PubMed: 23173055
WB Use at an assay dependent concentration. PubMed: 21156754

Target

  • FunctionCalcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
  • Tissue specificityDetected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta.
  • Involvement in diseaseFamilial arrhythmogenic right ventricular dysplasia 2
    Ventricular tachycardia, catecholaminergic polymorphic 1, with or without atrial dysfunction and/or dilated cardiomyopathy
  • Sequence similaritiesBelongs to the ryanodine receptor (TC 1.A.3.1) family. RYR2 subfamily.
    Contains 3 B30.2/SPRY domains.
    Contains 5 MIR domains.
  • Developmental stageExpressed in myometrium during pregnancy.
  • DomainThe calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm.
  • Post-translational
    modifications
    Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels.
    Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium.
  • Cellular localizationSarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARVC2 antibody
    • ARVD2 antibody
    • Cardiac muscle ryanodine receptor antibody
    • Cardiac muscle ryanodine receptor-calcium release channel antibody
    • hRYR-2 antibody
    • ryanodine receptor 2 (cardiac) antibody
    • Ryanodine receptor 2 antibody
    • RyR antibody
    • RYR-2 antibody
    • RyR2 antibody
    • RYR2_HUMAN antibody
    • Type 2 ryanodine receptor antibody
    • VTSIP antibody
    see all

Anti-Ryanodine receptor 2 (phospho S2808) antibody images

  • ab59225 (1/50 dilution) staining Ryanodine Receptor 2 in human brain tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunizing peptide.

    ab59225 (1/50 dilution) staining Ryanodine Receptor in human brain tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunizing peptide.

References for Anti-Ryanodine receptor 2 (phospho S2808) antibody (ab59225)

This product has been referenced in:
  • Beca S  et al. Phosphodiesterase type 3A regulates basal myocardial contractility through interacting with sarcoplasmic reticulum calcium ATPase type 2a signaling complexes in mouse heart. Circ Res 112:289-97 (2013). Read more (PubMed: 23168336) »
  • Huang Y  et al. Sarcoplasmic phospholamban protein is involved in the mechanisms of postresuscitation myocardial dysfunction and the cardioprotective effect of nitrite during resuscitation. PLoS One 8:e82552 (2013). WB ; Rat . Read more (PubMed: 24386101) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization Yes - 0.01% Triton X-100
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Oct 26 2016

Thank you for your reply.


We have a number of phospho specific antibodies in our catalog, but ab59225 is the only phospho specific antibody we have to Ryanodine Receptors.


In order to determine specifically which residue...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"