Anti-Ryanodine receptor 2 (phospho S2808) antibody (ab59225)

Overview

  • Product name
    Anti-Ryanodine receptor 2 (phospho S2808) antibody
    See all Ryanodine receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to Ryanodine receptor 2 (phospho S2808)
  • Specificity
    ab59225 detects endogenous levels of Ryanodine Receptor 2 only when phosphorylated at serine 2808.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Ryanodine receptor 2. Synthetic phosphopeptide derived from human Ryanodine Receptor around the phosphorylation site of serine 2808 (R-I-SP-Q-T).
    Database link: Q92736

  • Positive control
    • Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/5000.
WB Use at an assay dependent concentration. PubMed: 21156754

Target

  • Function
    Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
  • Tissue specificity
    Detected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta.
  • Involvement in disease
    Familial arrhythmogenic right ventricular dysplasia 2
    Ventricular tachycardia, catecholaminergic polymorphic 1, with or without atrial dysfunction and/or dilated cardiomyopathy
  • Sequence similarities
    Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR2 subfamily.
    Contains 3 B30.2/SPRY domains.
    Contains 5 MIR domains.
  • Developmental stage
    Expressed in myometrium during pregnancy.
  • Domain
    The calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm.
  • Post-translational
    modifications
    Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels.
    Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium.
  • Cellular localization
    Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARVC2 antibody
    • ARVD2 antibody
    • Cardiac muscle ryanodine receptor antibody
    • Cardiac muscle ryanodine receptor-calcium release channel antibody
    • hRYR-2 antibody
    • ryanodine receptor 2 (cardiac) antibody
    • Ryanodine receptor 2 antibody
    • RyR antibody
    • RYR-2 antibody
    • RyR2 antibody
    • RYR2_HUMAN antibody
    • Type 2 ryanodine receptor antibody
    • VTSIP antibody
    see all

Images

  • ab59225 (1/50 dilution) staining Ryanodine Receptor 2 in human brain tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunizing peptide.

References

This product has been referenced in:
  • Park SJ  et al. Specific Sirt1 Activator-mediated Improvement in Glucose Homeostasis Requires Sirt1-Independent Activation of AMPK. EBioMedicine 18:128-138 (2017). WB . Read more (PubMed: 28396013) »
  • Hu LR  et al. Deregulated Ca(2+) cycling underlies the development of arrhythmia and heart disease due to mutant obscurin. Sci Adv 3:e1603081 (2017). WB, IF ; Mouse . Read more (PubMed: 28630914) »

See all 8 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0.01% Triton X-100
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 26 2016

Thank you for your reply.


We have a number of phospho specific antibodies in our catalog, but ab59225 is the only phospho specific antibody we have to Ryanodine Receptors.


In order to determine specifically which residue...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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