The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 552 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Use at an assay dependent dilution.
FunctionCommunication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from SR following depolarization of T-tubules.
Tissue specificityBrain, skeletal muscle, placenta and possibly liver and kidney. In brain, highest levels are found in the cerebellum, hippocampus, caudate nucleus and amygdala, with lower levels in the corpus callosum, substantia nigra and thalamus.
Sequence similaritiesBelongs to the ryanodine receptor (TC 1.A.3.1) family. RYR3 subfamily. Contains 3 B30.2/SPRY domains. Contains 1 EF-hand domain. Contains 5 MIR domains.
References for Anti-Ryanodine Receptor 3 antibody - Carboxyterminal end (ab56000)
This product has been referenced in:
Nensa FM et al. Amyloid beta a4 precursor protein-binding family B member 1 (FE65) interactomics revealed synaptic vesicle glycoprotein 2A (SV2A) and sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) as new binding proteins in the human brain. Mol Cell Proteomics13:475-88 (2014).
Read more (PubMed: 24284412) »