Other Immunogen Type corresponding to Chicken Ryanodine Receptor. Partially purified chicken pectoral muscle ryanodine receptor.
Our Abpromise guarantee covers the use of ab2868 in the following tested applications.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||1/100 - 1/1000.|
|IP||Use at an assay dependent concentration.|
|WB||1/5000. Predicted molecular weight: 565 kDa. As RyR is a large protein, we recommend using a 6-8% gel, wet transfering protein overnight at low voltage, adding 0.1% SDS to transfer buffer and reducing methanol to 10% or less.|
|Inhibition Assay||Use at an assay dependent concentration.|
ab2868 staining Ryanodine Receptor (red) in Mouse Skeletal muscle cells at day 10 of agrin-treated differentation by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 10% serum for 45 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) for 2 hours at 20°C. An Alexa Fluor® 647-conjugated Donkey anti-goat IgG polyclonal (1/500) was used as the secondary antibody. Nucleus stained blue
Image from Blaich A et al., J Biol Chem 2012 Jun 29;287(27):22616-25. Epub 2012 May 15.; Fig 5.; doi: 10.1074/jbc.M111.335927; June 29, 2012 The Journal of Biological Chemistry, 287, 23216-23226.Representative Western blots for Ser(P)2814 and Ryanodine Receptor in control and Cav1.2I/E mutant mouse hearts. Ryanodine Receptor (bottom panel) was detected with ab2868.
ab2868 staining Ryanodine Receptor in Rat cardiomyocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1) and blocked with 3% BSA for 1 hour at 18°C. Samples were incubated with primary antibody (1/300 in PBS + 3% BSA) for 16 hours at 4°C. An Alexa Fluor® 546-conjugated Goat anti-mouse IgG (H+L) polyclonal (1/300) was used as the secondary antibody.