Overview

  • Product nameAnti-RYBP antibody
    See all RYBP primary antibodies
  • Description
    Rabbit polyclonal to RYBP
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    TPKGDMSAVNDESF

    , corresponding to amino acids 215-228 of Human DEDAF.

  • Positive control
    • A549, HepG2 or 3T3 whole cell lysate.
  • General notesStable for one year.


    Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesDEDAF Antibody is affinity chromatography purified via peptide column.
  • Primary antibody notesApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 32 kDa.Can be blocked with RYBP peptide (ab6097). Can be blocked with DEDAF peptide (human) .
IHC-P Use a concentration of 5 - 10 µg/ml.
ICC/IF Use a concentration of 1 - 20 µg/ml.

Target

  • FunctionMay be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. In tumor cell lines, may induce apoptosis.
  • Tissue specificityWidely expressed with highest levels in lymphoid tissues and placenta.
  • Sequence similaritiesContains 1 RanBP2-type zinc finger.
  • Cellular localizationNucleus. Cytoplasm. Primarily found in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410018J24Rik antibody
    • AAP 1 antibody
    • AAP1 antibody
    • APAP 1 antibody
    • APAP-1 antibody
    • APAP1 antibody
    • Apoptin associating protein 1 antibody
    • Apoptin-associating protein 1 antibody
    • cb337 antibody
    • Death effector domain associated factor antibody
    • Death effector domain-associated factor antibody
    • Death effector domain-associated factor B antibody
    • DED associated factor antibody
    • DED-associated factor antibody
    • DED-associated factor B antibody
    • DEDAF antibody
    • MGC62213 antibody
    • RING1 and YY1 binding protein a antibody
    • RING1 and YY1 binding protein antibody
    • RING1 and YY1-binding protein antibody
    • RING1 and YY1-binding protein B antibody
    • Ring1 interactor RYBP antibody
    • RYBP antibody
    • RYBP_HUMAN antibody
    • rybpa antibody
    • wu:fi34f03 antibody
    • YEAF 1 antibody
    • YEAF1 antibody
    • YY1 and E4TF1 associated factor 1 antibody
    • YY1 and E4TF1-associated factor 1 antibody
    • YY1- and E4TF1/GABP-associated factor 1 antibody
    • zgc:153842 antibody
    see all

Anti-RYBP antibody images

  • Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 µg/ml.

  • Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 µg /ml.

    Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 µg /ml.
  • ab5976 staining RYBP in Mouse embryonic stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.5% and blocked with 1% milk for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/400) was used as the secondary antibody.
  • Immunofluorescence of DEDAF in A549 cells using ab5976 at 20 ug/ml.

References for Anti-RYBP antibody (ab5976)

This product has been referenced in:
  • He J  et al. Kdm2b maintains murine embryonic stem cell status by recruiting PRC1 complex to CpG islands of developmental genes. Nat Cell Biol 15:373-84 (2013). Mouse . Read more (PubMed: 23502314) »

See 1 Publication for this product

Product Wall

Ich habe Rückmeldung aus dem Labor erhalten: Leider sieht es so aus, dass der ab128104 Anti-RYBP antibody [1B2] nur gegen das rekombinante Protein getestet wurde.

Soll ich Ihnen daher (wie besprochen) den ab107896 Anti-RYBP antibody zukommen...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Fixative Formaldehyde
Permeabilization Yes - 0.5 % triton
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Ligia Tavares

Verified customer

Submitted Sep 16 2011

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - whole cell (ES cells)
Specification ES cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step Real-time PCR
Username

Abcam user community

Verified customer

Submitted Mar 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (ES cells)
Loading amount 100000 cells
Specification ES cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Ligia Tavares

Verified customer

Submitted Mar 18 2011

Thank you again for sending the image of your blot. I contacted the originator of this antibody, and they have never seen that additional band at 60 kDa. You do seem to be detecting DEDAF as you are seeing a band at approx 32 kDa and most likely the ba...

Read More

Thank you for the details that you have submitted. From the description, it seems that you are detecting DEDAF at 32 kDa and are also seeing a non-specific band. I would suggest that you try decreasing the antibody concentration and/or the incubation p...

Read More

Thank you for your phone call. I contacted the originator of this antibody and they were able to provide me with the following information. First, they said that with the A549, HepG2 and 3T3 whole cell lysates, they only observed a band at 32 kDa (ther...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"