Anti-RYK antibody [EPR5169(2)] (ab124961)

Overview

  • Product nameAnti-RYK antibody [EPR5169(2)]
    See all RYK primary antibodies
  • Description
    Rabbit monoclonal [EPR5169(2)] to RYK
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to residues in Human RYK (intracellular domain).

  • Positive control
    • 293T, MCF7 and Jurkat cell lysates; Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

Target

  • FunctionMay be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
  • Tissue specificityObserved in all the tissues examined.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 protein kinase domain.
    Contains 1 WIF domain.
  • DomainThe extracellular WIF domain is responsible for Wnt binding.
  • Post-translational
    modifications
    Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Cleavage occurs during neuronal differentiation.
  • Cellular localizationMembrane. Nucleus. Cytoplasm. In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • D3S3195 antibody
    • ERK 3 antibody
    • Hydroxyaryl protein kinase antibody
    • JTK 5 antibody
    • JTK 5A antibody
    • JTK5 antibody
    • JTK5A antibody
    • JTK5A protein tyrosine kinase antibody
    • receptor like tyrosine kinase antibody
    • receptor like tyrosine kinase precursor antibody
    • RYK 1 antibody
    • Ryk antibody
    • RYK_HUMAN antibody
    • RYK1 antibody
    • Tyrosine protein kinase RYK antibody
    • Tyrosine protein kinase RYK precursor antibody
    • Tyrosine-protein kinase RYK antibody
    • Vik antibody
    see all

Anti-RYK antibody [EPR5169(2)] images

  • All lanes : Anti-RYK antibody [EPR5169(2)] (ab124961) at 1/50000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : MCF7 cell lysates
    Lane 3 : Jurkat cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 68 kDa
  • ab124961, at 1/50 dilution staining RYK in paraffin-embedded Human brain tissue, by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-RYK antibody [EPR5169(2)] (ab124961)

ab124961 has not yet been referenced specifically in any publications.

Product Wall

The antigen retrieval step is typically done after de-paraffinization and before any blocking steps. The aim is to partially reverse the effects of formaldehyde-based fixatives, to encourage recognition of the protein of interest by the antibody. The c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"