AbcamBiochemicals

(S)-(-)-Blebbistatin (ab120491)

Overview

  • Product name(S)-(-)-Blebbistatin
  • Description
    Selective myosin II ATPase activity inhibitor
  • Biological descriptionSelective myosin II ATPase activity inhibitor (IC50 = 8.5 μM). Active enantiomer of blebbistatin (ab120425). Inhibits actin-activated MgATPase activity and in vitro motility of class II myosins. Also inhibits contraction of the cytokinetic ring. Blocks cell blebbing and disrupts directed cell migration and cytokinesis.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120491 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    (S)-(-)-Blebbistatin images

    • ab32084 staining paxillin in MEF1 cells treated with (S)-(-)-Blebbistatin (ab120491), by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (S)-(-)-Blebbistatin , as described in literature.
      The cells were incubated at 37°C for 2h in media containing different concentrations of ab120491 ( (S)-(-)-Blebbistatin ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32084 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for (S)-(-)-Blebbistatin (ab120491)

    This product has been referenced in:
    • Takács B  et al. Myosin complexed with ADP and blebbistatin reversibly adopts a conformation resembling the start point of the working stroke. Proc Natl Acad Sci U S A 107:6799-804 (2010). Read more (PubMed: 20351242) »
    • Ivanov AI  et al. Myosin II regulates the shape of three-dimensional intestinal epithelial cysts. J Cell Sci 121:1803-14 (2008). Read more (PubMed: 18460584) »
    • Lucas-Lopez C  et al. The small molecule tool (S)-(-)-blebbistatin: novel insights of relevance to myosin inhibitor design. Org Biomol Chem 6:2076-84 (2008). Read more (PubMed: 18528569) »

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"